Peroxiredoxin V Reduces β-Lapachone-induced Apoptosis of Colon Cancer Cells

Peroxiredoxin (Prx) V has been known as an antioxidant enzyme which scavenges intracellular reactive oxygen species (ROS). Also, Prx V has been shown to mediate cell apoptosis in various cancers. However, the mechanism of Prx V-induced apoptosis in colon cancer cells remains unknown. Thus, in this s...

Full description

Saved in:
Bibliographic Details
Published in:Anticancer research 2019-07, Vol.39 (7), p.3677-3686
Main Authors: Liu, Yue, Kwon, Taeho, Kim, Ji-Su, Chandimali, Nisansala, Jin, Ying-Hua, Gong, Yi-Xi, Xie, Dan-Ping, Han, Ying-Hao, Jin, Mei-Hua, Shen, Gui-Nan, Jeong, Dong Kee, Lee, Dong-Sun, Cui, Yu-Dong, Sun, Hu-Nan
Format: Article
Language:English
Subjects:
Annexin V
Antioxidants
Apoptosis
Cancer
Cell Line, Tumor
Colon
Colon - metabolism
Colon cancer
Colonic Neoplasms - metabolism
Colorectal cancer
Flow cytometry
Fluorescence
Fluorescence microscopy
Humans
Lapachone
Naphthoquinones
Peroxiredoxin
Peroxiredoxins - physiology
Reactive oxygen species
Reactive Oxygen Species - metabolism
Scavenging
Signal transduction
Signaling
Western blotting
Wnt protein
Wnt Signaling Pathway
β-Catenin
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Peroxiredoxin (Prx) V has been known as an antioxidant enzyme which scavenges intracellular reactive oxygen species (ROS). Also, Prx V has been shown to mediate cell apoptosis in various cancers. However, the mechanism of Prx V-induced apoptosis in colon cancer cells remains unknown. Thus, in this study we analyzed the effects of Prx V in β-lapachone-induced apoptosis in SW480 human colon cancer cells. β-lapachone-induced apoptosis was analyzed by the MTT assay, western blotting, fluorescence microscopy, Annexin V staining and flow cytometry. Overexpression of Prx V, significantly decreased β-lapachone-induced cellular apoptosis and Prx V silencing increased β-lapachone-induced cellular apoptosis via modulating ROS scavenging activity compared to mock SW480 cells. In addition, to further explore the mechanism of Prx V regulated β-lapachone-induced SW480 cells apoptosis, the Wnt/β-catenin signaling was studied. The Wnt/ β-catenin signaling pathway was found to be induced by β-lapachone. Prx V regulates SW480 cell apoptosis via scavenging ROS cellular levels and mediating the Wnt/β-catenin signaling pathway, which was induced by β-lapachone.
ISSN:0250-7005
1791-7530
DOI:10.21873/anticanres.13516