The cell wall proteome from two strains of Pseudocercospora fijiensis with differences in virulence

• Published in: World journal of microbiology & biotechnology 2019-07, Vol.35 (7), p.105-19, Article 105
• Main Authors: Burgos-Canul, Yamily Y., Canto-Canché, Blondy, Berezovski, Maxim V., Mironov, Gleb, Loyola-Vargas, Víctor M., Barba de Rosa, Ana Paulina, Tzec-Simá, Miguel, Brito-Argáez, Ligia, Carrillo-Pech, Mildred, Grijalva-Arango, Rosa, Muñoz-Pérez, Gilberto, Islas-Flores, Ignacio
• Format: Article
• Language: English
• Subjects: Applied Microbiology; Ascomycota - genetics; Ascomycota - isolation & purification; Ascomycota - metabolism; Ascomycota - pathogenicity; Biochemistry; Bioinformatics; Biological activity; Biomedical and Life Sciences; Biotechnology; Black Sigatoka; Cell Wall - genetics; Cell Wall - metabolism; Cell walls; Contaminants; Contamination; Environmental Engineering/Biotechnology; Fungal Proteins - genetics; Fungal Proteins - metabolism; Fungi; Genes, Fungal - genetics; Genome, Fungal; High-performance liquid chromatography; Life Sciences; Liquid chromatography; Microbiology; Molecular modelling; Musa - microbiology; Nitrogen; Original Paper; Pathogenesis; Pathogenicity; Pathogens; Plant Diseases - microbiology; Plant Leaves - microbiology; Proteins; Proteome; Proteomics; Pseudocercospora fijiensis; Tandem Mass Spectrometry; Virulence; Virulence factors; Virulence Factors - genetics; Virulence Factors - metabolism
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-019-2681-2

Pseudocercospora fijiensis causes black Sigatoka disease, the most important threat to banana. The cell wall is crucial for fungal biological processes, including pathogenesis. Here, we performed cell wall proteomics analyses of two P. fijiensis strains, the highly virulent Oz2b, and the less virulent C1233 strains. Strains were starved from nitrogen to mimic the host environment. Interestingly, in vitro cultures of the C1233 strain grew faster than Oz2b in PDB medium, suggesting that C1233 survives outside the host better than the highly virulent Oz2b strain. Both strains were submitted to nitrogen starvation and the cell wall proteins were isolated and subjected to nano-HPLC–MS/MS. A total of 2686 proteins were obtained from which only 240 had a known function and thus, bioinformatics analyses were performed on this group. We found that 90 cell wall proteins were shared by both strains, 21 were unique for Oz2b and 39 for C1233. Shared proteins comprised 24 pathogenicity factors, including Avr4 and Ecp6, two effectors from P. fijiensis , while the unique proteins comprised 16 virulence factors in C1233 and 11 in Oz2b. The P. fijiensis cell wall proteome comprised canonical proteins, but thirty percent were atypical, a feature which in other phytopathogens has been interpreted as contamination. However, a comparison with the identities of atypical proteins in other reports suggests that the P. fijiensis proteins we detected were not contaminants. This is the first proteomics analysis of the P. fijiensis cell wall and our results expands the understanding of the fundamental biology of fungal phytopathogens and will help to decipher the molecular mechanisms of pathogenesis and virulence in P. fijiensis . Graphic abstract