The differential effect of clarithromycin and azithromycin on induction of macrolide resistance in Mycobacterium abscessus

Antibiotic resistance in renders treatment poorly effective. Despite -gene-mediated macrolide resistance, treatment with azithromycin or clarithromycin is recommended. It is contested whether macrolides differ in ) induction. We determine whether this is the case. CIP104536 was used. Minimum inhibit...

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Bibliographic Details
Published in:Future microbiology 2019-06, Vol.14 (9), p.749-755
Main Authors: Schildkraut, Jodie A, Pennings, Lian J, Ruth, Mike M, de Brouwer, Arjan Pm, Wertheim, Heiman Fl, Hoefsloot, Wouter, de Jong, Arjan, van Ingen, Jakko
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Antibiotic resistance
Antibiotics
Azithromycin
Azithromycin - pharmacology
Clarithromycin
Clarithromycin - pharmacology
Clinical outcomes
Cystic fibrosis
Drug Resistance, Bacterial - drug effects
Gene Expression Profiling
Infections
Kinetics
Macrolides - pharmacology
Microbial Sensitivity Tests
Mycobacterium abscessus
Mycobacterium abscessus - drug effects
Ribonucleic acid
RNA
RNA, Bacterial - analysis
RNA, Messenger - analysis
Transcriptional Activation - drug effects
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Summary:Antibiotic resistance in renders treatment poorly effective. Despite -gene-mediated macrolide resistance, treatment with azithromycin or clarithromycin is recommended. It is contested whether macrolides differ in ) induction. We determine whether this is the case. CIP104536 was used. Minimum inhibitory concentrations of clarithromycin and azithromycin were determined. Time-kill kinetics of exposed to azithromycin or clarithromycin were performed and RNA was isolated at predetermined intervals for quantification. Minimum inhibitory concentrations increased >30-fold. Time-kill kinetics showed a temporary bacteriostatic effect, abrogated by induced resistance. expression was increased following exposure to either macrolide for 7 days. Both macrolides induce resistance similarly, and this should not be an argument in choosing either macrolide for therapy.
ISSN:1746-0913
1746-0921
DOI:10.2217/fmb-2018-0310