Recent Advances of Molecular Optical Probes in Imaging of β‑Galactosidase

β-Galactosidase (β-Gal), as a lysosomal hydrolytic enzyme, plays an important physiological role in catalyzing the hydrolysis of glycosidic bonds which convert lactose into galactose. Moreover, upregulation of β-Gal is often correlated with the occurrence of primary ovarian cancers and cell senescen...

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Bibliographic Details
Published in:Bioconjugate chemistry 2019-08, Vol.30 (8), p.2089-2101
Main Authors: Zhang, Jianjian, Cheng, Penghui, Pu, Kanyi
Format: Article
Language:English
Subjects:
Bioluminescence
Chemiluminescence
Fluorescence
Fluorescent indicators
Galactose
Galactosidase
Lactose
Medical imaging
Organic chemistry
Ovarian cancer
Probes
Senescence
Temporal resolution
β-Galactosidase
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Summary:β-Galactosidase (β-Gal), as a lysosomal hydrolytic enzyme, plays an important physiological role in catalyzing the hydrolysis of glycosidic bonds which convert lactose into galactose. Moreover, upregulation of β-Gal is often correlated with the occurrence of primary ovarian cancers and cell senescence. Thereby, detection of β-Gal activity is relevant to cancer diagnosis. Optical imaging possesses high spatial and temporal resolution, high sensitivity, and real-time imaging capability. These properties are beneficial for the detection of β-Gal in living systems. This Review summarizes the recent progress in development of molecular optical probes for near-infrared fluorescence (NIRF), bioluminescence (BL), chemiluminescence (CL), or photoacoustic (PA) imaging of β-Gal in biological systems. The challenges and opportunities in the probe design for detection of β-Gal are also discussed.
ISSN:1043-1802
1520-4812
DOI:10.1021/acs.bioconjchem.9b00391