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Determination of sialic acid levels by using surface‐enhanced Raman spectroscopy in periodontitis and gingivitis
Objectives To compare the sialic acid (SA) levels in saliva among periodontitis‐affected, gingivitis and control patients. Methods The study involved 93 subjects. The participants were divided into three groups: (1) 30 subjects without periodontal disease (control group); (2) 30 subjects with gingiv...
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| Published in: | Oral diseases 2019-09, Vol.25 (6), p.1627-1633 |
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| container_title | Oral diseases |
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| creator | Hernández‐Cedillo, Alondra García-Valdivieso, Ma. Guadalupe Hernández‐Arteaga, Aida Catalina Patiño-Marín, Nuria Vértiz-Hernández, Ángel Antonio José‐Yacamán, Miguel Navarro‐Contreras, Hugo Ricardo |
| description | Objectives
To compare the sialic acid (SA) levels in saliva among periodontitis‐affected, gingivitis and control patients.
Methods
The study involved 93 subjects. The participants were divided into three groups: (1) 30 subjects without periodontal disease (control group); (2) 30 subjects with gingivitis; and (3) 33 subjects with periodontitis. The oral parameters examined were as follows: (a) Simplified Oral Hygiene Index; (b) Calculus Index; (c) Gingival Index; (d) probing pocket depth; and (e) level of epithelial attachment. SA levels in saliva were measured by means of surface‐enhanced Raman spectroscopy (SERS). This method has demonstrated the capacity to detect extremely low concentrations of molecules. The spectrum was calibrated using analytical reagent SA.
Results
The obtained median values for SA concentrations were 5.98, 7.32, and 17.12 mg/dl for control, gingivitis, and periodontitis patients, respectively.
Conclusions
Our measurements by SERS corroborate that in periodontitis‐affected patients, the SA concentration is larger than their concentrations in either control or gingivitis patients. This confirms previous reports and opens the possibility of using SERS as a diagnostic tool. |
| doi_str_mv | 10.1111/odi.13141 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2252286347</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2252286347</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3531-865cda0f14f2c0b59b93acc5c1d3b75d130bef4775c6792d5867bc8339779e5a3</originalsourceid><addsrcrecordid>eNp1kcFKHjEQx4O0qLU9-AIS6MUeVjPJZrM5FrWtIAilhd6WbDKrkd1kTXaV79ZH6DP2SRr72R4KncvMwG_-zMyfkENgJ1DiNDp_AgJq2CH70DCoWMvli1ILWVeSi2975FXOd4yB0oLvkj0BoJTU9T5J57hgmnwwi4-BxoFmb0ZvqbHe0REfcMy039A1-3BD85oGY_Hn9x8Ybk2w6OhnM5lA84x2STHbOG-oD3TG5KOLYfGLz9QER2_KvH94al-Tl4MZM755zgfk64eLL2efqqvrj5dn768qK6SAqm2kdYYNUA_csl7qXgtjrbTgRK-kA8F6HOpyh22U5k62jeptK4RWSqM04oAcb3XnFO9XzEs3-WxxHE3AuOaOc8l524haFfTtP-hdXFMo2xVKawDZyrZQ77aULZfmhEM3Jz-ZtOmAdU9GdMWI7rcRhT16Vlz7Cd1f8s_nC3C6BR79iJv_K3XX55dbyV89ipQY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2299115858</pqid></control><display><type>article</type><title>Determination of sialic acid levels by using surface‐enhanced Raman spectroscopy in periodontitis and gingivitis</title><source>Wiley-Blackwell Read & Publish Collection</source><creator>Hernández‐Cedillo, Alondra ; García-Valdivieso, Ma. Guadalupe ; Hernández‐Arteaga, Aida Catalina ; Patiño-Marín, Nuria ; Vértiz-Hernández, Ángel Antonio ; José‐Yacamán, Miguel ; Navarro‐Contreras, Hugo Ricardo</creator><creatorcontrib>Hernández‐Cedillo, Alondra ; García-Valdivieso, Ma. Guadalupe ; Hernández‐Arteaga, Aida Catalina ; Patiño-Marín, Nuria ; Vértiz-Hernández, Ángel Antonio ; José‐Yacamán, Miguel ; Navarro‐Contreras, Hugo Ricardo</creatorcontrib><description>Objectives
To compare the sialic acid (SA) levels in saliva among periodontitis‐affected, gingivitis and control patients.
Methods
The study involved 93 subjects. The participants were divided into three groups: (1) 30 subjects without periodontal disease (control group); (2) 30 subjects with gingivitis; and (3) 33 subjects with periodontitis. The oral parameters examined were as follows: (a) Simplified Oral Hygiene Index; (b) Calculus Index; (c) Gingival Index; (d) probing pocket depth; and (e) level of epithelial attachment. SA levels in saliva were measured by means of surface‐enhanced Raman spectroscopy (SERS). This method has demonstrated the capacity to detect extremely low concentrations of molecules. The spectrum was calibrated using analytical reagent SA.
Results
The obtained median values for SA concentrations were 5.98, 7.32, and 17.12 mg/dl for control, gingivitis, and periodontitis patients, respectively.
Conclusions
Our measurements by SERS corroborate that in periodontitis‐affected patients, the SA concentration is larger than their concentrations in either control or gingivitis patients. This confirms previous reports and opens the possibility of using SERS as a diagnostic tool.</description><identifier>ISSN: 1354-523X</identifier><identifier>EISSN: 1601-0825</identifier><identifier>DOI: 10.1111/odi.13141</identifier><identifier>PMID: 31177594</identifier><language>eng</language><publisher>Denmark: Wiley Subscription Services, Inc</publisher><subject>Adult ; Ag nanoparticles ; Dental calculus ; Dentistry ; Female ; Gingival index ; Gingivitis ; Gum disease ; Humans ; Male ; Middle Aged ; N-Acetylneuraminic Acid - analysis ; N-Acetylneuraminic Acid - chemistry ; Oral hygiene ; Periodontal diseases ; Periodontal Index ; Periodontitis ; Periodontitis - diagnosis ; Raman spectroscopy ; Saliva ; Saliva - chemistry ; Saliva - metabolism ; sialic acid ; Spectrum analysis ; Spectrum Analysis, Raman ; surface‐enhanced Raman</subject><ispartof>Oral diseases, 2019-09, Vol.25 (6), p.1627-1633</ispartof><rights>2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. All rights reserved</rights><rights>2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. All rights reserved.</rights><rights>Copyright © 2019 John Wiley & Sons A/S</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3531-865cda0f14f2c0b59b93acc5c1d3b75d130bef4775c6792d5867bc8339779e5a3</citedby><cites>FETCH-LOGICAL-c3531-865cda0f14f2c0b59b93acc5c1d3b75d130bef4775c6792d5867bc8339779e5a3</cites><orcidid>0000-0003-0333-0024</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31177594$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hernández‐Cedillo, Alondra</creatorcontrib><creatorcontrib>García-Valdivieso, Ma. Guadalupe</creatorcontrib><creatorcontrib>Hernández‐Arteaga, Aida Catalina</creatorcontrib><creatorcontrib>Patiño-Marín, Nuria</creatorcontrib><creatorcontrib>Vértiz-Hernández, Ángel Antonio</creatorcontrib><creatorcontrib>José‐Yacamán, Miguel</creatorcontrib><creatorcontrib>Navarro‐Contreras, Hugo Ricardo</creatorcontrib><title>Determination of sialic acid levels by using surface‐enhanced Raman spectroscopy in periodontitis and gingivitis</title><title>Oral diseases</title><addtitle>Oral Dis</addtitle><description>Objectives
To compare the sialic acid (SA) levels in saliva among periodontitis‐affected, gingivitis and control patients.
Methods
The study involved 93 subjects. The participants were divided into three groups: (1) 30 subjects without periodontal disease (control group); (2) 30 subjects with gingivitis; and (3) 33 subjects with periodontitis. The oral parameters examined were as follows: (a) Simplified Oral Hygiene Index; (b) Calculus Index; (c) Gingival Index; (d) probing pocket depth; and (e) level of epithelial attachment. SA levels in saliva were measured by means of surface‐enhanced Raman spectroscopy (SERS). This method has demonstrated the capacity to detect extremely low concentrations of molecules. The spectrum was calibrated using analytical reagent SA.
Results
The obtained median values for SA concentrations were 5.98, 7.32, and 17.12 mg/dl for control, gingivitis, and periodontitis patients, respectively.
Conclusions
Our measurements by SERS corroborate that in periodontitis‐affected patients, the SA concentration is larger than their concentrations in either control or gingivitis patients. This confirms previous reports and opens the possibility of using SERS as a diagnostic tool.</description><subject>Adult</subject><subject>Ag nanoparticles</subject><subject>Dental calculus</subject><subject>Dentistry</subject><subject>Female</subject><subject>Gingival index</subject><subject>Gingivitis</subject><subject>Gum disease</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>N-Acetylneuraminic Acid - analysis</subject><subject>N-Acetylneuraminic Acid - chemistry</subject><subject>Oral hygiene</subject><subject>Periodontal diseases</subject><subject>Periodontal Index</subject><subject>Periodontitis</subject><subject>Periodontitis - diagnosis</subject><subject>Raman spectroscopy</subject><subject>Saliva</subject><subject>Saliva - chemistry</subject><subject>Saliva - metabolism</subject><subject>sialic acid</subject><subject>Spectrum analysis</subject><subject>Spectrum Analysis, Raman</subject><subject>surface‐enhanced Raman</subject><issn>1354-523X</issn><issn>1601-0825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kcFKHjEQx4O0qLU9-AIS6MUeVjPJZrM5FrWtIAilhd6WbDKrkd1kTXaV79ZH6DP2SRr72R4KncvMwG_-zMyfkENgJ1DiNDp_AgJq2CH70DCoWMvli1ILWVeSi2975FXOd4yB0oLvkj0BoJTU9T5J57hgmnwwi4-BxoFmb0ZvqbHe0REfcMy039A1-3BD85oGY_Hn9x8Ybk2w6OhnM5lA84x2STHbOG-oD3TG5KOLYfGLz9QER2_KvH94al-Tl4MZM755zgfk64eLL2efqqvrj5dn768qK6SAqm2kdYYNUA_csl7qXgtjrbTgRK-kA8F6HOpyh22U5k62jeptK4RWSqM04oAcb3XnFO9XzEs3-WxxHE3AuOaOc8l524haFfTtP-hdXFMo2xVKawDZyrZQ77aULZfmhEM3Jz-ZtOmAdU9GdMWI7rcRhT16Vlz7Cd1f8s_nC3C6BR79iJv_K3XX55dbyV89ipQY</recordid><startdate>201909</startdate><enddate>201909</enddate><creator>Hernández‐Cedillo, Alondra</creator><creator>García-Valdivieso, Ma. Guadalupe</creator><creator>Hernández‐Arteaga, Aida Catalina</creator><creator>Patiño-Marín, Nuria</creator><creator>Vértiz-Hernández, Ángel Antonio</creator><creator>José‐Yacamán, Miguel</creator><creator>Navarro‐Contreras, Hugo Ricardo</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0333-0024</orcidid></search><sort><creationdate>201909</creationdate><title>Determination of sialic acid levels by using surface‐enhanced Raman spectroscopy in periodontitis and gingivitis</title><author>Hernández‐Cedillo, Alondra ; García-Valdivieso, Ma. Guadalupe ; Hernández‐Arteaga, Aida Catalina ; Patiño-Marín, Nuria ; Vértiz-Hernández, Ángel Antonio ; José‐Yacamán, Miguel ; Navarro‐Contreras, Hugo Ricardo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3531-865cda0f14f2c0b59b93acc5c1d3b75d130bef4775c6792d5867bc8339779e5a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adult</topic><topic>Ag nanoparticles</topic><topic>Dental calculus</topic><topic>Dentistry</topic><topic>Female</topic><topic>Gingival index</topic><topic>Gingivitis</topic><topic>Gum disease</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>N-Acetylneuraminic Acid - analysis</topic><topic>N-Acetylneuraminic Acid - chemistry</topic><topic>Oral hygiene</topic><topic>Periodontal diseases</topic><topic>Periodontal Index</topic><topic>Periodontitis</topic><topic>Periodontitis - diagnosis</topic><topic>Raman spectroscopy</topic><topic>Saliva</topic><topic>Saliva - chemistry</topic><topic>Saliva - metabolism</topic><topic>sialic acid</topic><topic>Spectrum analysis</topic><topic>Spectrum Analysis, Raman</topic><topic>surface‐enhanced Raman</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hernández‐Cedillo, Alondra</creatorcontrib><creatorcontrib>García-Valdivieso, Ma. Guadalupe</creatorcontrib><creatorcontrib>Hernández‐Arteaga, Aida Catalina</creatorcontrib><creatorcontrib>Patiño-Marín, Nuria</creatorcontrib><creatorcontrib>Vértiz-Hernández, Ángel Antonio</creatorcontrib><creatorcontrib>José‐Yacamán, Miguel</creatorcontrib><creatorcontrib>Navarro‐Contreras, Hugo Ricardo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Oral diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hernández‐Cedillo, Alondra</au><au>García-Valdivieso, Ma. Guadalupe</au><au>Hernández‐Arteaga, Aida Catalina</au><au>Patiño-Marín, Nuria</au><au>Vértiz-Hernández, Ángel Antonio</au><au>José‐Yacamán, Miguel</au><au>Navarro‐Contreras, Hugo Ricardo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of sialic acid levels by using surface‐enhanced Raman spectroscopy in periodontitis and gingivitis</atitle><jtitle>Oral diseases</jtitle><addtitle>Oral Dis</addtitle><date>2019-09</date><risdate>2019</risdate><volume>25</volume><issue>6</issue><spage>1627</spage><epage>1633</epage><pages>1627-1633</pages><issn>1354-523X</issn><eissn>1601-0825</eissn><abstract>Objectives
To compare the sialic acid (SA) levels in saliva among periodontitis‐affected, gingivitis and control patients.
Methods
The study involved 93 subjects. The participants were divided into three groups: (1) 30 subjects without periodontal disease (control group); (2) 30 subjects with gingivitis; and (3) 33 subjects with periodontitis. The oral parameters examined were as follows: (a) Simplified Oral Hygiene Index; (b) Calculus Index; (c) Gingival Index; (d) probing pocket depth; and (e) level of epithelial attachment. SA levels in saliva were measured by means of surface‐enhanced Raman spectroscopy (SERS). This method has demonstrated the capacity to detect extremely low concentrations of molecules. The spectrum was calibrated using analytical reagent SA.
Results
The obtained median values for SA concentrations were 5.98, 7.32, and 17.12 mg/dl for control, gingivitis, and periodontitis patients, respectively.
Conclusions
Our measurements by SERS corroborate that in periodontitis‐affected patients, the SA concentration is larger than their concentrations in either control or gingivitis patients. This confirms previous reports and opens the possibility of using SERS as a diagnostic tool.</abstract><cop>Denmark</cop><pub>Wiley Subscription Services, Inc</pub><pmid>31177594</pmid><doi>10.1111/odi.13141</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-0333-0024</orcidid></addata></record> |
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| identifier | ISSN: 1354-523X |
| ispartof | Oral diseases, 2019-09, Vol.25 (6), p.1627-1633 |
| issn | 1354-523X 1601-0825 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2252286347 |
| source | Wiley-Blackwell Read & Publish Collection |
| subjects | Adult Ag nanoparticles Dental calculus Dentistry Female Gingival index Gingivitis Gum disease Humans Male Middle Aged N-Acetylneuraminic Acid - analysis N-Acetylneuraminic Acid - chemistry Oral hygiene Periodontal diseases Periodontal Index Periodontitis Periodontitis - diagnosis Raman spectroscopy Saliva Saliva - chemistry Saliva - metabolism sialic acid Spectrum analysis Spectrum Analysis, Raman surface‐enhanced Raman |
| title | Determination of sialic acid levels by using surface‐enhanced Raman spectroscopy in periodontitis and gingivitis |
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