Direct effects of poly(ε-caprolactone) lipid-core nanocapsules on human immune cells

Poly(ε-caprolactone) lipid-core nanocapsules (LNCs) are efficient drug carriers and drug-free LNCs display therapeutic effects, inhibiting tumor growth and neutrophil activities. Herein, we investigated the direct actions of LNCs on human immune cells, to guide their therapeutic application. LNC’s u...

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Bibliographic Details
Published in:Nanomedicine (London, England) England), 2019-06, Vol.14 (11), p.1429-1442
Main Authors: Sandri, Silvana, Hebeda, Cristina Bichels, Loiola, Rodrigo Azevedo, Calgaroto, Selma, Uchiyama, Mayara Klimuk, Araki, Koiti, Frank, Luiza Abrahão, Paese, Karina, Guterres, Silvia Stanisçuaski, Pohlmann, Adriana Raffin, Farsky, Sandra Helena Poliselli
Format: Article
Language:English
Subjects:
Blood Cells - drug effects
Cancer therapies
Cell adhesion & migration
Cell cycle
cell migration
Cell Movement
Cell Proliferation
Cell Survival
Cytokines
Cytokines - metabolism
cytokines release
Drug Carriers - chemistry
Drug Carriers - metabolism
Extracellular Signal-Regulated MAP Kinases - metabolism
Gene Expression Regulation
Hexoses - chemistry
Human Umbilical Vein Endothelial Cells - drug effects
Humans
Immune system
immunosuppression
Kinases
Lipids
Lipids - chemistry
MAPK signaling
Melanoma
Nanocapsules - chemistry
Nanoparticles
Neutrophils
peripheral blood mononuclear cells
Polyesters - chemistry
polymorphonuclear cells
Signal Transduction
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Summary:Poly(ε-caprolactone) lipid-core nanocapsules (LNCs) are efficient drug carriers and drug-free LNCs display therapeutic effects, inhibiting tumor growth and neutrophil activities. Herein, we investigated the direct actions of LNCs on human immune cells, to guide their therapeutic application. LNC’s uptake, cytokine release, cell migration, proliferation and intracellular pathways under inflammatory stimulation were investigated. LNCs quickly penetrated leukocytes without cytotoxicity; inhibited mitogen-induced lymphocyte proliferation, cytokine release and leukocyte migration under inflammatory stimulation, which were associated with inhibition of the MAP kinase pathway and intracellular calcium influx. Hence, we showed LNCs as a down-regulatory agent on immune cells, suggesting that either the particles themselves or their application as a drug carrier can halt non-desired inflammatory processes.
ISSN:1743-5889
1748-6963
DOI:10.2217/nnm-2018-0484