Resonance Rayleigh Scattering as a Tool for Isoelectric Point Monitoring and Iron(III) Cation Determination

A resonance Rayleigh scattering (RRS) technique was utilized as a tool for isoelectric point monitoring and iron(III) cation determination. The spectral properties of some amphoteric molecules (proteins and a DNA sequence) were investigated using the RRS technique. When the pH values were kept at ar...

Full description

Saved in:
Bibliographic Details
Published in:Analytical Sciences 2019/10/10, Vol.35(10), pp.1149-1153
Main Authors: LI, Na, LIU, Shi Gang, HE, Yong Qin, MAI, Xi
Format: Article
Language:English
Subjects:
Analytical Chemistry
Cations
Chemistry
Deoxyribonucleic acid
DNA
DNA sequence
Iron
iron(III) detection
isoelectric point
Isoelectric points
Monitoring
Nucleotide sequence
pH effects
proteins
Rayleigh scattering
Resonance
Resonance Rayleigh scattering
Resonance scattering
Signal processing
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A resonance Rayleigh scattering (RRS) technique was utilized as a tool for isoelectric point monitoring and iron(III) cation determination. The spectral properties of some amphoteric molecules (proteins and a DNA sequence) were investigated using the RRS technique. When the pH values were kept at around their isoelectric points, especially high RRS signals could be obtained, which were much stronger than those at other pH values. By using the C30 DNA sequence as a probe, the iron(III) cation can be detected rapidly. After iron(III) was added to a C30 solution, a significantly decreased RRS signal was obtained. The sensing process can be finished within 10 min with a detection limit of 0.9 μM. Thus, a sensitive, selective, and label-free method was successfully developed for iron(III) detection.
ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.19P177