Comprehensive analysis of differences of N6-methyladenosine RNA methylomes between high-fat-fed and normal mouse livers

Aim: To assess the m6A methylome in mouse fatty liver induced by a high-fat diet (HFD). Materials & methods: MeRIP-seq was performed to identify differences in the m6A methylomes between the normal liver and fatty liver induced by an HFD. Results: As compared with the control group, the upmethyl...

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Bibliographic Details
Published in:Epigenomics 2019-08, Vol.11 (11), p.1267-1282
Main Authors: Luo, Zupeng, Zhang, Zhiwang, Tai, Lina, Zhang, Lifang, Sun, Zheng, Zhou, Lei
Format: Article
Language:English
Subjects:
Binding
DNA methylation
Fatty liver
Genes
Genomes
Glucose
High fat diet
Identification methods
Library collections
Lipid metabolism
Lipids
Liver
Liver diseases
Metabolism
N6-methyladenosine
Obesity
Ontology
Proteins
Ribonucleic acid
RNA
RNA-binding protein
Software
Splicing
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Summary:Aim: To assess the m6A methylome in mouse fatty liver induced by a high-fat diet (HFD). Materials & methods: MeRIP-seq was performed to identify differences in the m6A methylomes between the normal liver and fatty liver induced by an HFD. Results: As compared with the control group, the upmethylated coding genes upon feeding an HFD were primarily enriched in processes associated with lipid metabolism, while genes with downmethylation were enriched in processes associated with metabolism and translation. Furthermore, many RNA-binding proteins that potentially bind to differentially methylated m6A sites were mainly annotated in processes of RNA splicing. Conclusion: These findings suggest that differential m6A methylation may act on functional genes through RNA-binding proteins to regulate the metabolism of lipids in fatty liver disease.
ISSN:1750-1911
1750-192X
DOI:10.2217/epi-2019-0009