Biomass‐dependent emission of environmental DNA in jack mackerel Trachurus japonicus juveniles

Environmental DNA (eDNA) from juvenile jack mackerel Trachurus japonicus was detected in tanks with 1, 3, 10, or 30 individuals per tank. Quantitative PCR using a set of species‐specific primers and a probe revealed that the concentration of eDNA increased almost linearly with the density of fish. T...

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Bibliographic Details
Published in:Journal of fish biology 2019-09, Vol.95 (3), p.979-981
Main Authors: Horiuchi, Tomoya, Masuda, Reiji, Murakami, Hiroaki, Yamamoto, Satoshi, Minamoto, Toshifumi
Format: Article
Language:English
Subjects:
Animals
Biomass
carangidae
Deoxyribonucleic acid
DNA
Environmental DNA
Environmental DNA - chemistry
Fish
Fishes - physiology
Freshwater
Freshwater fish
Inland water environment
Juveniles
Mackerel
marine fish biomass estimation
Marine fishes
Nucleotide sequence
PCR
Primers
Real-Time Polymerase Chain Reaction
real‐time quantitative PCR
species‐specific primers and probe
Tanks
Trachurus japonicus
Water - chemistry
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Summary:Environmental DNA (eDNA) from juvenile jack mackerel Trachurus japonicus was detected in tanks with 1, 3, 10, or 30 individuals per tank. Quantitative PCR using a set of species‐specific primers and a probe revealed that the concentration of eDNA increased almost linearly with the density of fish. The coefficient of determination (R2) in the linear regression was lower than values previously reported for freshwater fishes in similar settings.
ISSN:0022-1112
1095-8649
DOI:10.1111/jfb.14095