Morphological Characterization and Gene Expression Patterns for Melanin Pigmentation in Rex Rabbit

Animal melanin has an important role in the formation of animal fur and skin, which is determined by its quantities, character, and distribution. To identify the effect of melanin on the formation of multi-colored Rex rabbits (Black, Chinchilla, Beaver, Protein cyan, Protein yellow, White), the stru...

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Bibliographic Details
Published in:Biochemical genetics 2019-10, Vol.57 (5), p.734-744
Main Authors: Hu, Shuaishuai, Zhai, Pin, Chen, Yang, Zhao, Bohao, Yang, Naisu, Wang, Manman, Xiao, Yeyi, Bao, Guolian, Wu, Xinsheng
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Biomedicine
Color
Coloration
Correlation analysis
Epidermis
Follicles
Fur-bearing animals
Gene expression
Genes
Hair
Human Genetics
Levels
Medical Microbiology
Melanin
Morphology
Original Article
Pigmentation
Protein structure
Proteins
Rabbits
Skin
Staining
Zoology
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Summary:Animal melanin has an important role in the formation of animal fur and skin, which is determined by its quantities, character, and distribution. To identify the effect of melanin on the formation of multi-colored Rex rabbits (Black, Chinchilla, Beaver, Protein cyan, Protein yellow, White), the structure of hair follicles and melanin content in multi-colored Rex rabbit skins were observed by Hematoxylin and Eosin (H&E) staining and melanin staining, respectively. The melanin granules were primarily found in the epidermis and hair follicle roots. The melanin content of skin was measured by extracting melanin from skin tissue. The results demonstrated that the melanin content was the highest in the skin of black Rex rabbit. Additionally, we measured the mRNA and protein expression levels of melanin-related key genes (MITF and TYR) in the skin of different hair color by quantitative real-time PCR and Wes assay, respectively. The results revealed that the mRNA expression levels in the skin of black Rex rabbit was highly expressed when as compared with other Rex rabbit skin ( P 
ISSN:0006-2928
1573-4927
DOI:10.1007/s10528-019-09929-x