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Berberine improves intestinal epithelial tight junctions by upregulating A20 expression in IBS-D mice

•Berberine protects intestinal epithelial tight junctions and repairs the increasing intestinal permeability of IBS-D caused by the intestinal epithelial barrier.•Berberine reduces the expression of TNF-α and inhibits the activation of the NF-κB-MLCK pathway.•Berberine improves intestinal epithelial...

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Bibliographic Details
Published in:Biomedicine & pharmacotherapy 2019-10, Vol.118, p.109206-109206, Article 109206
Main Authors: Hou, Qiuke, Zhu, Shuilian, Zhang, Changrong, Huang, Yongquan, Guo, Yajuan, Li, Peiwu, Chen, Xinlin, Wen, Yi, Han, Quanbin, Liu, Fengbin
Format: Article
Language:English
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Summary:•Berberine protects intestinal epithelial tight junctions and repairs the increasing intestinal permeability of IBS-D caused by the intestinal epithelial barrier.•Berberine reduces the expression of TNF-α and inhibits the activation of the NF-κB-MLCK pathway.•Berberine improves intestinal epithelial tight junction integrity in a mouse model of IBS-D through the expression of A20. To investigate effects of berberine exerts on A20 expression and regulation of intestinal epithelial tight junctions via the TNF-α-NF-κB-MLCK pathway in Diarrhea-Predominant Irritable Bowel Syndrome (IBS-D). C57BL/6 wild type (WT) and A20 IEC-KO mice (48 each) were randomly divided into normal control (NC), model control (MC), rifaximin and berberine groups (12 mice per group). An experimental model of IBS-D was established using 4% acetic acid and evaluated by haematoxylin-eosin (HE) staining. rifaximin and berberine mice were treated with rifaximin and berberine, respectively. Intestinal epithelial space of WT berberine mice improved more than A20 IEC-KO berberine mice compared to MC mice. WT berberine mice exhibited greater expression of A20 compared with MC mice(P 
ISSN:0753-3322
1950-6007
DOI:10.1016/j.biopha.2019.109206