The facile and simple synthesis of poly(3,4ethylenedioxythiophene) anchored reduced graphene oxide nanocomposite for biochemical analysis

• Published in: Analytica chimica acta 2019-10, Vol.1077, p.150-159
• Main Authors: Dinesh, Bose, Vilian, A.T. Ezhil, Kwak, Cheol Hwan, Huh, Yun Suk, Saraswathi, Ramiah, Han, Young-Kyu
• Format: Article
• Language: English
• Subjects: Ascorbic acid; Biochemical analysis; Buffer solutions; Calibration; Detection limits; Dopamine; Electrochemical analysis; Electrochemical impedance spectroscopy; Electrochemistry; Graphene; Nanocomposites; PEDOT; Photoelectron spectroscopy; Photoelectrons; Physicochemical properties; Raman spectroscopy; Reduced graphene oxide; Simultaneous determination; Spectroscopy; Spectrum analysis; Uric acid; Voltammetry
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2019.05.053

In this article, we demonstrate the potentiostatic electrodeposition of poly(3,4-ethylenedioxythiophene) (PEDOT) on reduced graphene oxide (RGO) to develop a nanocomposite-modified electrode that separates three coexisting biofluids – ascorbic acid (AA), dopamine (DA), and uric acid (UA) – in a 0.1 M Phosphate buffer solution at a physiological pH (7.4). The texture, physicochemical properties, and electrochemical behavior of the PEDOT-RGO were explored using UV–visible spectroscopy, Raman spectroscopy, X-ray photoelectron spectroscopy, electron microscopic techniques, and electrochemical impedance spectroscopy (EIS). A PEDOT-RGO/GCE was evaluated with respect to a bare GCE, RGO/GCE, and PEDOT/GCE for the simultaneous sensing of AA, DA, and UA. The difference in voltammetric peak potentials was about 180 mV between AA and DA and 120 mV between DA and UA. The differential pulse voltammetric sensor provided a linear calibration for a wide concentration range (0.1–907 μM for AA, 0.1–901 μM for DA, and 0.1–701 μM for UA) with detection limits of 1.5 μM, 0.6 μM, and 0.2 μM for AA, DA, and UA, respectively. The developed sensor was validated by the detection of AA, DA, and UA in a vitamin C tablet, a dopamine hydrochloride injection, and human serum samples. [Display omitted] •A nanocomposite PEDOT-RGO modified GCE is employed for the simultaneous detection of AA, DA, and UA.•DPV method is used to the detection of AA, DA, and UA in physiological condition with high selectivity.•A good peak to peak separation, wide linear ranges, and low detection limit in μM level.•Excellent real sample performance of AA, DA, and UA in pharmaceutical drugs and human serum samples.