Lateral root initiation requires the sequential induction of transcription factors LBD16 and PUCHI in Arabidopsis thaliana

Lateral root (LR) formation in Arabidopsis thaliana is initiated by asymmetric division of founder cells, followed by coordinated cell proliferation and differentiation for patterning new primordia. The sequential developmental processes of LR formation are triggered by a localized auxin response. L...

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Bibliographic Details
Published in:The New phytologist 2019-10, Vol.224 (2), p.749-760
Main Authors: Goh, Tatsuaki, Toyokura, Koichi, Yamaguchi, Nobutoshi, Okamoto, Yoshie, Uehara, Takeo, Kaneko, Shutaro, Takebayashi, Yumiko, Kasahara, Hiroyuki, Ikeyama, Yoshifumi, Okushima, Yoko, Nakajima, Keiji, Mimura, Tetsuro, Tasaka, Masao, Fukaki, Hidehiro
Format: Article
Language:English
Subjects:
Arabidopsis - genetics
Arabidopsis - growth & development
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Arabidopsis thaliana
auxin
Gene Expression Regulation, Developmental - physiology
Gene Expression Regulation, Plant - physiology
Indoleacetic Acids - metabolism
lateral root
LBD16
Plant Roots - growth & development
PUCHI
Transcription Factors - genetics
Transcription Factors - metabolism
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Summary:Lateral root (LR) formation in Arabidopsis thaliana is initiated by asymmetric division of founder cells, followed by coordinated cell proliferation and differentiation for patterning new primordia. The sequential developmental processes of LR formation are triggered by a localized auxin response. LATERAL ORGAN BOUNDARIES-DOMAIN 16 (LBD16), an auxin-inducible transcription factor, is one of the key regulators linking auxin response in LR founder cells to LR initiation. We identified key genes for LR formation that are activated by LBD16 in an auxin-dependent manner. LBD16 targets identified include the transcription factor gene PUCHI, which is required for LR primordium patterning. We demonstrate that LBD16 activity is required for the auxin-inducible expression of PUCHI. We show that PUCHI expression is initiated after the first round of asymmetric cell division of LR founder cells and that premature induction of PUCHI during the preinitiation phase disrupts LR primordium formation. Our results indicate that LR initiation requires the sequential induction of transcription factors LBD16 and PUCHI.
ISSN:0028-646X
1469-8137
DOI:10.1111/nph.16065