Fatty Acid Content and Profile of the Novel Strain of Coccomyxa elongata (Trebouxiophyceae, Chlorophyta) Cultivated at Reduced Nitrogen and Phosphorus Concentrations

• Published in: Journal of phycology 2019-10, Vol.55 (5), p.1154-1165
• Main Authors: Maltsev, Yevhen, Maltseva, Irina, Maltseva, Svetlana, Kociolek, John Patrick, Kulikovskiy, Maxim, Henley, W.
• Format: Article
• Language: English
• Subjects: Biomass; Cell size; Chlorophyta; Coccomyxa; Cultivation; DNA; Elongation; fatty acid profile; Fatty Acids; Freshwater; Inland water environment; Linolenic acid; lipid; Microalgae; Nitrogen; nitrogen and phosphorus deprivation; Oleic acid; Palmitic acid; Phosphates; Phosphorus; Phylogenetics; Phylogeny; Profiles; Protein structure; Secondary structure; Trebouxiophyceae; Vegetative cells
• ISSN: 0022-3646; 1529-8817
• DOI: 10.1111/jpy.12903

A novel freshwater strain of Coccomyxa elongata (MZ–Ch64) was isolated from the Zaporizhia region, Ukraine. The identification was based on the phylogenetic analysis of SSU rDNA gene and ITS1–5.8S rDNA–ITS2 region and predicted secondary structure of the ITS2. Phylogenetic analysis placed this strain in the Coccomyxa group, within the class Trebouxiophyceae. The novel strain MZ–Ch64 formed a strongly supported lineage closest with C. elongata. The MZ–Ch64 strain differed from the morphological description of the species by the size of vegetative cells and absence of small mucilaginous caps at one end of the cell. A number of experiments with different concentrations of phosphate and nitrate were conducted to evaluate changes in the resulting fatty acid profiles and biomass productivity. The fatty acid profile and total fatty acids varied significantly under different nutrient deficiencies. The dominant fatty acid during cultivation on standard BBM medium, as well as in phosphorus‐depleted conditions, was oleic acid (to 48.0%–54.6% of total fatty acids). Absence of nitrogen alone, and absence of both nitrogen and phosphorus, led to an increase of palmitic acid (to 24.7%–25.6%), cis‐7‐hexadecenoic acid (to 14.8%) and α‐linolenic acid (to 9.1%–10.1%) in comparison with the control sample. The greatest variation was found for oleic acid (31.9%–54.6%). Thus, this strain can be considered as a potential producer of oleic acid or cis‐7‐hexadecenoic and α‐linolenic acids for biotechnological applications.