Ambient temperature‐mediated enzymic activities and intestinal microflora in Lymantria dispar larvae

• Published in: Archives of insect biochemistry and physiology 2019-10, Vol.102 (2), p.e21597-n/a
• Main Authors: Zeng, JianYong, Shi, ZhongBin, Shi, JianHong, Guo, JiaXing, Zhang, GuoCai, Zhang, Jie
• Format: Article
• Language: English
• Subjects: Abundance; Ambient temperature; antioxidant enzyme; Antioxidants; Bacteria; Butterflies & moths; Carboxylesterase; Catalase; Caterpillars; digestive enzyme; Enzymatic activity; Enzymes; Firmicutes; Glutathione; gut microbiota; Herbivores; Intestinal microflora; Intestine; Larvae; Lipase; Lymantria dispar; Peroxidase; Pest control; Polyphenol oxidase; Proteobacteria; Stress; Superoxide dismutase; Temperature
• ISSN: 0739-4462; 1520-6327
• DOI: 10.1002/arch.21597

To understand how ambient temperature affect the gypsy moth larvae, and provide a theoretical basis for pest control in different environments. Fourth instar gypsy moth larvae were incubating for 3 hr at 15℃, 20℃, 25℃, 30℃, 35℃, and 40℃, respectively. Afterward, digestive and antioxidant enzyme activities, total antioxidant capacity, and intestinal microflora community were analyzed to reveal how the caterpillars respond to ambient temperature stress. Results showed that both digestive and antioxidant enzymes were regulated by the ambient temperature. The optimum incubation temperatures of protease, amylase, trehalase, and lipase in gypsy moth larvae were 30℃, 25℃, and 20℃, respectively. When the incubation temperature was deviated optimum temperatures, digestive enzyme activities would be downregulated depending on the extent of temperature stress. In addition, glutathione S‐transferase, peroxidase, catalase, and polyphenol oxidase would be activated under a sufferable temperature stress, but superoxide dismutase and carboxylesterase (CarE) would be inhibited. In addition, results showed that the top two abundant phyla were Proteobacteria and Firmicutes. The phylum Firmicutes abundance was decreased and phylum Proteobacteria abundance was increased by ambient temperature stress. Moreover, it suggested that gypsy moth caterpillars at different ambient temperature mainly differed from each other by Escherichia‐Shigella and Bifidobacterium in control, Acinetobacter in T15, and Lactobacillus in T40, respectively. The representative enzymes and the gut microbiota in Lymantria dispar at different aliment temperature.