Intracellular localization of histone deacetylase HDA6 in plants

• Published in: Journal of plant research 2019-09, Vol.132 (5), p.629-640
• Main Authors: Kurita, Kazuki, Sakamoto, Yuki, Naruse, Sota, Matsunaga, Tomoko M., Arata, Hideyuki, Higashiyama, Tetsuya, Habu, Yoshiki, Utsumi, Yoshinori, Utsumi, Chikako, Tanaka, Maho, Takahashi, Satoshi, Kim, Jong-Myong, Seki, Motoaki, Sakamoto, Takuya, Matsunaga, Sachihiro
• Format: Article
• Language: English
• Subjects: Acetic acid; Acetylation; Arabidopsis; Arabidopsis - metabolism; Arabidopsis Proteins - metabolism; Biomedical and Life Sciences; Cassava; Cell Nucleus - metabolism; Cereal crops; Chromatin; Drought; Drought resistance; Droughts; Dynamic control; Eukaryotes; Fusion protein; Gene Expression Profiling; Gene silencing; Heterochromatin; Histone acetyltransferase; Histone deacetylase; Histone Deacetylase 6 - metabolism; Histone Deacetylases - metabolism; Histones; Intracellular; Life Sciences; Localization; Manihot - metabolism; Meristems; Oryza; Oryza - metabolism; Plant Biochemistry; Plant Ecology; Plant Physiology; Plant Roots - metabolism; Plant Sciences; Regular Paper; Stress, Physiological - genetics
• ISSN: 0918-9440; 1618-0860
• DOI: 10.1007/s10265-019-01124-8

Histone modification is an important epigenetic mechanism in eukaryotes. Histone acetyltransferase and deacetylase regulate histone acetylation levels antagonistically, leading to dynamic control of chromatin structure. One of the histone deacetylases, HDA6, is involved in gene silencing in the heterochromatin regions, chromocenter formation, and metabolic adaptation under drought stress. Although HDA6 plays an important role in chromatin control and response to drought stress, its intracellular localization has not been observed in detail. In this paper, we generated transformants expressing HDA6-GFP in the model plant, Arabidopsis thaliana , and the crops, rice, and cassava. We observed the localization of the fusion protein and showed that HDA6-GFP was expressed in the whole root and localized at the nucleus in Arabidopsis, rice, and cassava. Remarkably, HDA6-GFP clearly formed speckles that were actively colocalized with chromocenters in Arabidopsis root meristem. In contrast, such speckles were unlikely to be formed in rice or cassava. Because AtHDA6 directly binds to the acetate synthesis genes, which function in drought tolerance, we performed live imaging analyses to examine the cellular dynamics of pH in roots and the subnuclear dynamics of AtHDA6 responding to acetic acid treatment. The number of HDA6 speckles increased during drought stress, suggesting a role in contributing to drought stress tolerance.