An integrative overview of physiological and proteomic changes of cytokinin‐induced potato (Solanum tuberosum L.) tuber development in vitro

• Published in: Physiologia plantarum 2020-03, Vol.168 (3), p.675-693
• Main Authors: Cheng, Lixiang, Wang, Dongxia, Wang, Yuping, Xue, Hongwei, Zhang, Feng
• Format: Article
• Language: English
• Subjects: Benzyl Compounds - pharmacology; Biological activity; Chaperones; Cytokinins; Cytokinins - physiology; Electrophoresis; Gel electrophoresis; Gene Expression Regulation, Plant; Glycolysis; Homeostasis; Image analysis; Image processing; Metabolites; Molecular modelling; Physiology; Plant hormones; Plant Proteins - physiology; Plant Tubers - growth & development; Potatoes; Protein folding; Proteins; Proteome; Proteomes; Proteomics; Purines - pharmacology; Renewable energy; Solanum tuberosum - physiology; Starch; Transcription; Translation; Transport
• ISSN: 0031-9317; 1399-3054
• DOI: 10.1111/ppl.13014

Potato tuberization is a complicated biological process regulated by multiple phytohormones, in particular cytokinins (CKs). The information available on the molecular mechanisms regulating tuber development by CKs remains largely unclear. Physiological results initially indicated that low 6‐benzylaminopurine (BAP) concentration (3 mg l−1) advanced the tuberization beginning time and promoted tuber formation. A comparative proteomics approach was applied to investigate the proteome change of tuber development by two‐dimensional gel electrophoresis in vitro, subjected to exogenous BAP treatments (0, 3, 6 and 13 mg l−1). Quantitative image analysis showed a total of 83 protein spots with significantly altered abundance (>2.5‐fold, P