Papaver somniferum L. mediated novel bioinspired lead oxide (PbO) and iron oxide (Fe2O3) nanoparticles: In-vitro biological applications, biocompatibility and their potential towards HepG2 cell line

• Published in: Materials Science & Engineering C 2019-10, Vol.103, p.109740-109740, Article 109740
• Main Authors: Muhammad, Wali, Khan, Muhammad Aslam, Nazir, Munazza, Siddiquah, Aisha, Mushtaq, Sadaf, Hashmi, Syed Salman, Abbasi, Bilal Haider
• Format: Article
• Language: English
• Subjects: alpha-Amylases - antagonists & inhibitors; alpha-Amylases - metabolism; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Anticancer; Anticancer properties; Antifungal Agents - chemistry; Antifungal Agents - pharmacology; Antioxidants; Antioxidants - chemistry; Antioxidants - pharmacology; Biocompatibility; Biosynthesized; Chemical composition; Cytotoxicity; Drug Evaluation, Preclinical; Drug Stability; Erythrocytes - drug effects; Ferric Compounds - chemistry; Fourier transforms; Free radicals; Hemolytic Agents - chemistry; Hemolytic Agents - pharmacology; Hep G2 Cells; Humans; Hypoglycemic Agents - chemistry; Hypoglycemic Agents - pharmacology; Infrared spectroscopy; Iron oxide NPs; Iron oxides; Lead; Lead - chemistry; Lead oxide NPs; Lead oxides; Materials science; Metal Nanoparticles - chemistry; Metal Nanoparticles - therapeutic use; Nanoparticles; Organic chemistry; Oxides - chemistry; Papaver - chemistry; Papaver - metabolism; Papaver somniferum; Papaver somniferum L; Scanning electron microscopy; Scavenging; Spectroscopy, Fourier Transform Infrared; X-Ray Diffraction; α-Amylase
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2019.109740

To overcome the disadvantages of chemical and physical methods, phyto-fabricated nanoparticles attained great attention due to their multifarious applications. Here we successfully demonstrated Papaver somniferum L. mediated green synthesis of lead oxide (PbO) and iron oxide (Fe2O3) nanoparticles. Characterization of nanoparticles involved techniques including X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and energy dispersive X-ray (EDX) associated with scanning electron microscopy (SEM). XRD analysis confirmed the phase identification and crystalline nature. FTIR analysis confirmed the capping of nanoparticles by plants' phytochemicals. SEM revealed morphological features of PbO and Fe2O3 with size of nanoparticles being 23 ± 11 nm and 38 ± 13 nm, respectively. The elemental composition of the nanoparticles was confirmed by EDX. Both bacterial and fungal isolates showed susceptibility towards PbO and Fe2O3 NPs. Both the NPs also showed considerable total antioxidant potential, free radical scavenging potential and reducing power. Insignificant level of α-amylase for both NPs was observed. Fe2O3 NPs showed superior biocompatibility with human RBCs as compared to PbO whereas PbO showed more potent anti-cancer activity as compared to Fe2O3 NPs. Overall our study concluded that both NPs played vital role in multiple biological assays however, extensive research focused on cytotoxic evaluation of NPs in-vivo is required. [Display omitted] •Novel biological synthesis of PbO NPs and Fe2O3 NPs using pod of Papaver somniferum L.•Characterization (XRD, FT-IR, SEM, EDX) of the biosynthesized NPs.•PbO NPs and Fe2O3 NPs exhibited broad spectrum antibacterial and antifungal activities.•Fe2O3 NPs was found more compatible with freshly isolated RBCs.•Superior anticancer activity of PbO NPs was observed against HepG2 Cell line