Rolling "wool-balls": rapid live-cell mapping of membrane sialic acids via poly-p-benzoquinone/ethylenediamine nanoclusters

Here, we report a convenient, fast labeling strategy for the imaging of cell surface sialic acids (SAs, nine-carbon monosaccharides located at the terminals of cell surface sugar chains). This strategy is based on the synthesis of sticky, furry and fluorescent "wool-balls", which are wound...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2019-08, Vol.55 (65), p.9681-9684
Main Authors: Chen, Bin-Bin, Wang, Xiao-Yuan, Qian, Ruo-Can
Format: Article
Language:English
Subjects:
Aldehydes
Animals
Benzoquinones - chemical synthesis
Benzoquinones - chemistry
Cell Line, Tumor
Density functional theory
Ethylenediamine
Ethylenediamines - chemical synthesis
Ethylenediamines - chemistry
Fluorescence
Fluorescent Dyes - chemistry
Humans
Mapping
Mice
Microscopy, Fluorescence - methods
Monosaccharides
Nanoclusters
Nanoparticles - chemistry
Neuraminidase - chemistry
Particle Size
Polymers - chemical synthesis
Polymers - chemistry
Quinones
RAW 264.7 Cells
Schiff Bases - chemical synthesis
Schiff Bases - chemistry
Sialic Acids - analysis
Sialic Acids - chemistry
Wool
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Summary:Here, we report a convenient, fast labeling strategy for the imaging of cell surface sialic acids (SAs, nine-carbon monosaccharides located at the terminals of cell surface sugar chains). This strategy is based on the synthesis of sticky, furry and fluorescent "wool-balls", which are wound into nanoclusters from p-benzoquinone/ethylenediamine polymer "wires". With abundant amino groups at the surface, the wool-balls can easily stick to the C-7 aldehyde group generated at the ends of periodate treated SAs in less than 30 min.
ISSN:1359-7345
1364-548X
DOI:10.1039/c9cc03338f