Delivery of antisense peptide nucleic acid by gold nanoparticles for the inhibition of virus replication

We aim to use peptide nucleic acid (PNA) for antisense therapy against bovine viral diarrhea virus (BVDV), a surrogate model of human hepatitis C virus, and introduce an optimal approach for delivering PNA into the cell. PNA was designed for hybridization to the 5′-untranslated region of BVDV RNA in...

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Bibliographic Details
Published in:Nanomedicine (London, England) England), 2019-07, Vol.14 (14), p.1827-1840
Main Authors: Ghaffari, Elnaz, Rezatofighi, Seyedeh Elham, Ardakani, Mohammad Roayaei, Rastegarzadeh, Saadat
Format: Article
Language:English
Subjects:
antisense
antiviral activity
bovine viral diarrhea virus
Cell adhesion & migration
Cell culture
Cytotoxicity
Deoxyribonucleic acid
Design
Diarrhea
DNA
Genomes
Gold
gold nanoparticles
Hepatitis
hepatitis C virus
HIV
Human immunodeficiency virus
Nanoparticles
peptide nucleic acid
Peptides
Spectrum analysis
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Summary:We aim to use peptide nucleic acid (PNA) for antisense therapy against bovine viral diarrhea virus (BVDV), a surrogate model of human hepatitis C virus, and introduce an optimal approach for delivering PNA into the cell. PNA was designed for hybridization to the 5′-untranslated region of BVDV RNA in order to form a heteroduplex structure and inhibit the translation and replication of virus. Gold nanoparticles (AuNPs) were used as a delivery system for PNA. The cellular uptake of PNA-AuNPs and inhibition of BVDV infection in the middle stage of viral replication were found. Further research is warranted to develop AuNPs as a potential vehicle for delivering PNA in order to remove viruses from the infected cells.
ISSN:1743-5889
1748-6963
DOI:10.2217/nnm-2018-0520