Biodistribution and toxickinetic variances of chemical and green Copper oxide nanoparticles in vitro and in vivo

• Published in: Journal of trace elements in medicine and biology 2019-09, Vol.55, p.154-169
• Main Authors: Dey, Aditi, Manna, Subhankar, Adhikary, Jaydeep, Chattopadhyay, Sourav, De, Sriparna, Chattopadhyay, Dipankar, Roy, Somenath
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis - drug effects; Apoptotic protein; Biochemical toxicity; Biodistribution; Cell Survival - drug effects; Cells, Cultured; Copper - chemistry; Copper - pharmacokinetics; Copper oxide nanoparticle; Cytokine; Dose-Response Relationship, Drug; Humans; Kinetics; Lymphocytes - drug effects; Lymphocytes - metabolism; Mice; Mice, Inbred BALB C; Nanoparticles - chemistry; Nanotoxicology; Reactive Oxygen Species - metabolism; Tissue Distribution
• ISSN: 0946-672X; 1878-3252
• DOI: 10.1016/j.jtemb.2019.06.012

The proposed toxic, biodistribution and immunological response difference of S1NPs and S2NPs based on this study data. [Display omitted] •Synthesis and characterization of green and chemical CuONPs were performed.•Chemical CuONPs induced greater ROS generation and apoptosis than green CuONPs in vitro.•Both NPs altered cytokines and apoptotic protein levels in vitro and in vivo.•Biodistribution, elimination and histology in case of both NPs indicated toxicity. In this study, chemical (S1) and green (S2) Copper Oxide nanoparticles (NPs) were synthesized to determine their biodistribution and toxicokinetic variances in vitro and in vivo. Both NPs significantly released Copper ions (Cu) in lymphocytes and were primarily deposited in the mononuclear phagocyte system (MPS) such as the liver and spleen in mice. In particular, S2NPs seemed to be prominently stored in the spleen, whereas the S1NPs were widely stored in more organs including the liver, heart, lungs, kidney and intestine. The circulation in the blood and fecal excretions both showed higher S2NPs contents respectively. Measurements of cell viability, Hemolysis assay, Reactive Oxygen Species (ROS) generation, biochemical estimation and apoptotic or necrotic study in lymphocytes after 24 h and measurements of body and organ weight, serum chemistry evaluation, cytokines level, protein expressions and histopathology of Balb/C mice after 15 days indicated significant toxicity difference between the S1NPs and S2NPs. Our observations proved that the NPs physiochemical properties influence toxicity and Biodistribution profiles in vitro and in vivo.