Efficacy of Andrographis paniculata supplements induce a non-specific immune system against the pathogenicity of Aeromonas hydrophila infection in Indian major carp (Labeo rohita)

• Published in: Environmental science and pollution research international 2020-07, Vol.27 (19), p.23420-23436
• Main Authors: Palanikani, Radhakrishnan, Chanthini, Kanagaraj Muthu-Pandian, Soranam, Ramaiah, Thanigaivel, Annamalai, Karthi, Sengodan, Senthil-Nathan, Sengottayan, Murugesan, Arunachalam Ganesan
• Format: Article
• Language: English
• Subjects: Aeromonas hydrophila; Andrographis; Animals; Aquaculture; Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Carps; Disease control; Drug resistance; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; Environmental science; Fish Diseases; Geophysical methods; Geophysics; Gram-Negative Bacterial Infections; Graphical methods; Groundwater; Groundwater chemistry; Heavy metals; Hemoglobin; Histology; Immune System; Multivariate statistical analysis; Organic chemistry; Parameter estimation; Pathogens; Plant extracts; Pollutant Removal and the Health Effects of Environmental Pollution; Salt water intrusion; Seawater; Statistical analysis; Statistical methods; Sustainable development; Swimming; Virulence; Waste Water Technology; Water Management; Water Pollution Control; Water resources
• ISSN: 0944-1344; 1614-7499
• DOI: 10.1007/s11356-019-05957-7

Aeromonas hydrophila , an opportunistic fish pathogen, which causes several major diseases including skin ulcer and haemorrhagic septicemia, contributes considerably to the lethality in aquaculture. Chemical and antibiotic treatment employed against A. hydrophila for disease management are expensive and consequently prompted the advent of drug resistance among the pathogens. To overcome these draw backs, alternative aquatic disease control methods using conventional plant-based medicines are focussed. Our present study aimed to augment the fish non-specific immune system with the implementation of methanolic crude extracts of Andrographis paniculata to Labeo rohita , for evaluating their efficacy against A. hydrophila. Histology of major organs of A. hydrophila -infected fish such as the gills and liver displayed severe tissue damage. A. paniculata extracts exhibited the strong antibacterial activity against A. hydrophila even at lower concentrations (50 μl). The extracts also altered the haematological profile of treated infected fishes by increasing the levels of haemoglobin and total erythrocyte-leucocyte counts, along with the phagocytic index. The extracts also had a significant impact on modifying the anatomy and swimming pattern of infected fish, post treatment with the extracts. Also, A. paniculata treated infected fishes in all the plant extract administration methods, viz. injection, oral feeding and diffusion, and reduced the cumulative mortality rate to less than 30%. Even lower concentrations of A. paniculata extracts (50 μl) resulted in maximum relative percentage survival of treated fishes. Therefore, our findings suggest that A. paniculata was effective against A. hydrophila infection in aquaculture, thereby maintaining a healthy status of these fishes in aquaculture.