Expression of MicroRNAs miR-145, miR-181c, miR-199a and miR-1183 in the Blood and Hippocampus of Patients with Mesial Temporal Lobe Epilepsy

• Published in: Journal of molecular neuroscience 2019-12, Vol.69 (4), p.580-587
• Main Authors: Antônio, Luana Grupioni Lourenço, Freitas-Lima, Priscila, Pereira-da-Silva, Gabriela, Assirati, João Alberto, Matias, Caio Marconato, Cirino, Mucio Luiz Assis, Tirapelli, Luis Fernando, Velasco, Tonicarlo Rodrigues, Sakamoto, Americo Ceiki, Carlotti, Carlos Gilberto, Tirapelli, Daniela Pretti da Cunha
• Format: Article
• Language: English
• Subjects: Adult; Autopsies; Biomarkers; Biomarkers - blood; Biomarkers - metabolism; Biomedical and Life Sciences; Biomedicine; Blood; Cell Biology; Diagnosis; Epilepsy; Epilepsy, Temporal Lobe - blood; Epilepsy, Temporal Lobe - diagnosis; Epilepsy, Temporal Lobe - genetics; Epilepsy, Temporal Lobe - metabolism; Female; Hippocampus; Hippocampus - metabolism; Humans; Male; MicroRNAs; MicroRNAs - blood; MicroRNAs - genetics; MicroRNAs - metabolism; Middle Aged; miRNA; Necropsy; Neurochemistry; Neurology; Neurosciences; Proteomics; Sclerosis; Statistical methods; Surgical outcomes; Temporal lobe
• ISSN: 0895-8696; 1559-1166
• DOI: 10.1007/s12031-019-01386-w

The aim of this study was to analyze the expression profiles of the microRNAs (miRNAs) miR-145, miR-181c, miR-199a and miR-1183 in the hippocampus and blood of patients with mesial temporal lobe epilepsy with hippocampal sclerosis (MTLE-HS) and to investigate whether these can be used as diagnosis and prognosis biomarkers for epilepsy. Hippocampus and blood samples were collected from 20 patients with MTLE-HS, ten of whom had a favorable surgical outcome (Engel I) and ten with an unfavorable surgical outcome (Engel III-IV). Hippocampus samples from autopsied individuals with no neurological or psychiatric medical history (necropsy samples) and blood samples from healthy individuals were used as controls. Real-time quantitative PCR (RQ-PCR) was used to analyze miRNA expression. The results showed that the expressions of these miRNAs differed quantitatively in the hippocampus and blood of patients with MTLE-HS in comparison to the respective control. This difference was most pronounced for miR-145, which was hypo-expressed in the hippocampus and hyper-expressed in the blood of MTLE-HS patients. MiRNAs miR-145, miR-181c, miR-199a and miR-1183 were hyper-expressed in the blood of patients with MTLE-HS. No statistical differences in the levels of these miRNAs in the blood or hippocampus were found between Engel I patients and Engel III-IV patients. These results suggest that the analyzed microRNAs are potential circulating biomarkers for epilepsy diagnosis.