Color-coded Imaging of the Fate of Cancer-cell-derived Exosomes During Pancreatic Cancer Metastases in a Nude-mouse Model

Tumor-derived exosomes play important roles in tumor metastases. In this report, we observed the fate of tumor-derived exosomes in pancreatic cancer metastatic nude-mouse models using color-coded imaging. Mia-PaCa-2 human pancreatic cancer cells expressing red fluorescent protein (RFP) were transduc...

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Bibliographic Details
Published in:Anticancer research 2019-08, Vol.39 (8), p.4055-4060
Main Authors: Satake, Tomoyuki, Suetsugu, Atsushi, Nakamura, Miki, Kunisada, Takahiro, Saji, Shigetoyo, Moriwaki, Hisataka, Shimizu, Masahito, Hoffman, Robert M
Format: Article
Language:English
Subjects:
Animal models
Animals
Bone marrow
Cancer
CD63 antigen
Cell Lineage - genetics
Color
Disease Models, Animal
Exosomes
Exosomes - genetics
Fluorescence
Green fluorescent protein
Green Fluorescent Proteins - chemistry
Humans
Kupffer cells
Liver Neoplasms - diagnostic imaging
Liver Neoplasms - pathology
Liver Neoplasms - secondary
Luminescent Proteins - chemistry
Lungs
Macrophages
Metastases
Metastasis
Mice
Neoplasm Metastasis
Pancreatic cancer
Pancreatic Neoplasms - diagnostic imaging
Pancreatic Neoplasms - genetics
Pancreatic Neoplasms - pathology
Proteins
Red Fluorescent Protein
Spleen
Tumor Microenvironment - drug effects
Tumors
Xenograft Model Antitumor Assays
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Summary:Tumor-derived exosomes play important roles in tumor metastases. In this report, we observed the fate of tumor-derived exosomes in pancreatic cancer metastatic nude-mouse models using color-coded imaging. Mia-PaCa-2 human pancreatic cancer cells expressing red fluorescent protein (RFP) were transduced by exosome-specific pCT-CD63-green fluorescent protein (GFP) and injected in the spleen of nude mice. Four weeks after injection of these cells into the spleen, liver metastases developed and tumor-derived exosomes were observed within the metastatic cancer cells and in Kupffer cells. Furthermore, tumor-derived exosomes diffused to bone marrow and lung cells, especially macrophages, without any metastases present. In the present study, we visualized the distribution of cancer-derived exosomes for the first time at the cellular level, in a pancreatic-cancer metastatic model.
ISSN:0250-7005
1791-7530
DOI:10.21873/anticanres.13561