A New Technique to Determine the Phosphate Oxygen Isotope Composition of Freshwater Samples at Low Ambient Phosphate Concentration

The oxygen isotope composition of dissolved inorganic phosphate (δ18Op) offers new opportunities to understand the sources and the fate of phosphorus (P) in freshwater ecosystems. However, current analytical protocols for determining δ18Op are unable to generate reliable data for samples in which am...

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Published in:Environmental science & technology 2019-09, Vol.53 (17), p.10288-10294
Main Authors: Tcaci, Marina, Barbecot, Florent, Hélie, Jean-Francois, Surridge, Ben W. J, Gooddy, Daren C
Format: Article
Language:English
Subjects:
Aquatic ecosystems
Biogeochemistry
Contamination
Freeze drying
Freshwater ecosystems
Isotope composition
Isotope ratios
Isotopes
Mass spectrometry
Mass spectroscopy
Oxygen
Oxygen isotopes
Phosphates
Phosphorus
Protocol
Rivers
Water analysis
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cited_by cdi_FETCH-LOGICAL-a505t-8d28e2540ee4acab85fef0a35df9b43aa51373afcf51e0b946a15d33466696963
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container_end_page 10294
container_issue 17
container_start_page 10288
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creator Tcaci, Marina
Barbecot, Florent
Hélie, Jean-Francois
Surridge, Ben W. J
Gooddy, Daren C
description The oxygen isotope composition of dissolved inorganic phosphate (δ18Op) offers new opportunities to understand the sources and the fate of phosphorus (P) in freshwater ecosystems. However, current analytical protocols for determining δ18Op are unable to generate reliable data for samples in which ambient P concentrations are extremely low, precisely the systems in which δ18Op may provide new and important insights into the biogeochemistry of P. In this Article, we report the development, testing and initial application of a new technique that enables δ18Op analysis to be extended into such ecosystems. The twist spinning mode (TSM) protocol described here enables >1000 L of sample with a P concentration
doi_str_mv 10.1021/acs.est.9b00631
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Combined with a new freeze-drying method to maximize the yield and minimize the contamination of silver phosphate generated for isotope ratio mass spectrometry, the TSM protocol is able to generate accurate and precise δ18Op data. We evaluated the TSM protocol using synthetic test solutions and subsequently applied the protocol to samples from locations around the Saint Lawrence River in Montreal, Canada. 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source American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list)
subjects Aquatic ecosystems
Biogeochemistry
Contamination
Freeze drying
Freshwater ecosystems
Isotope composition
Isotope ratios
Isotopes
Mass spectrometry
Mass spectroscopy
Oxygen
Oxygen isotopes
Phosphates
Phosphorus
Protocol
Rivers
Water analysis
title A New Technique to Determine the Phosphate Oxygen Isotope Composition of Freshwater Samples at Low Ambient Phosphate Concentration
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