In-vitro antigiardial activity and GC-MS analysis of Eucalyptus globulus and Zingiber officinalis essential oils against Giardia lamblia cysts in simulated condition to human’s body

In order to finding of potent natural medication/agent which can kill giardial cysts in the interval between lysing of outer membrane of cysts due to exposure by acidic condition of stomach and their encystation in proximal small intestine, In-vitro antigiardial activity and GC-MS analysis of Eucaly...

Full description

Saved in:
Bibliographic Details
Published in:Annals of parasitology 2019, Vol.65 (2), p.129-138
Main Authors: Dehghani-Samani, Azam, Madreseh-Ghahfarokhi, Samin, Dehghani-Samani, Amir, Pirali, Yaser
Format: Article
Language:English
Subjects:
Eucalyptus - chemistry
Gas Chromatography-Mass Spectrometry
Giardia lamblia - drug effects
Humans
Oils, Volatile - pharmacology
Zingiber officinale - chemistry
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In order to finding of potent natural medication/agent which can kill giardial cysts in the interval between lysing of outer membrane of cysts due to exposure by acidic condition of stomach and their encystation in proximal small intestine, In-vitro antigiardial activity and GC-MS analysis of Eucalyptus globulus and Zingiber officinalis against Giardia lamblia were studied in simulated condition to human’s body singly and in combination. Essential oils were extracted and their chemical components were identified via GC-MS method. After purification, cysts were exposure to acidic condition and were challenged by different concentrations of essential oils in simulated condition to human’s body. Percentages of inactivated (killed) cysts as efficacy of antigiardial activity were calculated and analyzed statistically. Presence of 1,8-eucalyptol, α-pinene, α-terpineol acetate, etc. in essential oil of E. globulus and presence of geraniol, α-zingiberene, (E,E)-α-farnesene, etc. in Z. officinalis essential oil were identified. Highest antigiardial activity (73.55%) was observed for eucalyptus essential oil in time 480 minutes after exposure. Efficacies of ginger and combined essential oils were different in different times. This study shows considerable antigiardial activity for both of essential oils singly and in combination together against giardial cysts. In-vivo study of protective effect of these essential oils against giardiasis can be considered as a subject for next studies.
ISSN:2299-0631
DOI:10.17420/ap6502.192