Proteomics insights into the effects of MSTN on muscle glucose and lipid metabolism in genetically edited cattle

• Published in: General and comparative endocrinology 2020-05, Vol.291, p.113237-113237, Article 113237
• Main Authors: Xin, Xiang-Bo, Yang, Shu-Ping, Li, Xin, Liu, Xin-Feng, Zhang, Lin-Lin, Ding, Xiang-Bin, Zhang, Sheng, Li, Guang-Peng, Guo, Hong
• Format: Article
• Language: English
• Subjects: Adenylate Kinase - metabolism; Adipose Tissue - metabolism; Amino Acid Sequence; AMPK; Animals; Animals, Genetically Modified; Cattle; Cattle - metabolism; Computational Biology; Gene Editing; Glucose - metabolism; Glycogen - metabolism; Lipid Metabolism; Metabolic cross-talk; Muscle, Skeletal - metabolism; Myostatin; Myostatin - metabolism; Peptides - chemistry; Peptides - metabolism; Phosphoproteins - metabolism; Phosphorylation; Proteomic; Proteomics; Reproducibility of Results; Signal Transduction
• ISSN: 0016-6480; 1095-6840
• DOI: 10.1016/j.ygcen.2019.113237

•Comparative proteomic and phosphoproteomic analyses of gluteus muscle tissues from MSTN−/− transgenic cattle were performed to explore the signaling pathway of MSTN in metabolic cross-talk and cellular metabolism.•A total of 72 differentially expressed proteins (DEPs) and 36 differentially expressed phosphoproteins (DEPPs) were identified in MSTN−/− cattle compared to WT cattle based on the TMT6-plex labeling method.•The DEPs were categorized as muscle, translation and lipid metabolism related proteins along with mitochondrial activity associated proteins, while the DEPPs were mainly associated with glycol-metabolism pathway.•The fatty acid β-oxidation process and glycolysis pathway were increased and the content of intramuscular fat and glycogen were decreased in MSTN−/− cattle.•MSTN knockout triggers the activation of AMPK signaling pathways to regulate glucose and lipid metabolism by increasing the AMP/ATP ratio. The molecular mechanism underlying myostatin (MSTN)-regulated metabolic cross-talk remains poorly understood. In this study, we performed comparative proteomic and phosphoproteomic analyses of gluteus muscle tissues from MSTN−/− transgenic cattle using a shotgun-based tandem mass tag (TMT) 6-plex labeling method to explore the signaling pathway of MSTN in metabolic cross-talk and cellular metabolism during muscle development. A total of 72 differentially expressed proteins (DEPs) and 36 differentially expressed phosphoproteins (DEPPs) were identified in MSTN−/− cattle compared to wild-type cattle. Bioinformatics analyses showed that MSTN knockout increased the activity of many key enzymes involved in fatty acid β-oxidation and glycolysis processes in cattle. Furthermore, comprehensive pathway analyses and hypothesis-driven AMP-activated protein kinase (AMPK) activity assays suggested that MSTN knockout triggers the activation of AMPK signaling pathways to regulate glucose and lipid metabolism by increasing the AMP/ATP ratio. Our results shed new light on the potential regulatory mechanism of MSTN associated with metabolic cross-talk in muscle development, which can be used in animal breeding to improve meat production in livestock animals, and can also provide valuable insight into treatments for obesity and diabetes mellitus in humans.