Adseverin modulates morphology and invasive function of MCF7 cells

Adseverin (Ads) is a Ca2+-dependent actin-capping and severing protein that is highly expressed in gastric, prostate and bladder cancer cells. Currently it is unknown whether Ads contributes to the subcortical actin remodeling associated with the formation of cell extensions and matrix invasion in c...

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Bibliographic Details
Published in:Biochimica et biophysica acta. Molecular basis of disease 2019-10, Vol.1865 (10), p.2716-2725
Main Authors: Tanic, J., Wang, Y., Lee, W., Coelho, N.M., Glogauer, M., McCulloch, C.A.
Format: Article
Language:English
Subjects:
Actin filaments
Actins - metabolism
Breast Neoplasms - metabolism
Cell migration
Cell Movement
Collagen - metabolism
Collagen degradation
CRISPR-Cas Systems
Fibronectins - metabolism
Gelsolin - genetics
Gelsolin - metabolism
Humans
MCF-7 Cells - metabolism
Phagocytosis
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Summary:Adseverin (Ads) is a Ca2+-dependent actin-capping and severing protein that is highly expressed in gastric, prostate and bladder cancer cells. Currently it is unknown whether Ads contributes to the subcortical actin remodeling associated with the formation of cell extensions and matrix invasion in cancer. We compared cell extension formation and matrix degradation in Ads wildtype and Ads-null MCF7 breast cancer cells generated by CRISPR/Cas9. Compared with wildtype, Ads-null cells plated on fibronectin or collagen exhibited a more circular morphology with shorter cell extensions (37% reduction on fibronectin; p 
ISSN:0925-4439
1879-260X
DOI:10.1016/j.bbadis.2019.07.015