Design, synthesis and biological evaluation of 4-aminoquinoline-guanylthiourea derivatives as antimalarial agents

• Published in: Bioorganic chemistry 2019-10, Vol.91, p.103094-103094, Article 103094
• Main Authors: Bhagat, Shweta, Arfeen, Minhajul, Das, Gourav, Ramkumar, Mridula, Khan, Shabana I., Tekwani, Babu L., Bharatam, Prasad V.
• Format: Article
• Language: English
• Subjects: 4-Aminoquinoline; Guanylthiourea; In vitro studies; Molecular docking; Molecular dynamics; PfDHFR
• ISSN: 0045-2068; 1090-2120
• DOI: 10.1016/j.bioorg.2019.103094

[Display omitted] •Structure based design of 4-aminoquinoline and guanylthiourea conjugated derivatives.•Synthesis of designed molecules as antimalarial agents.•In vitro studies resulted eight molecules with antimalarial activity against PfD6 and PfW2 strain.•Molecular dynamics simulations were performed on the most active molecule (4c). Guanylthiourea (GTU) has been identified as an important antifolate antimalarial pharmacophore unit, whereas, 4-amino quinolones are already known for antimalarial activity. In the present work molecules carrying 4-aminoquinoline and GTU moiety have been designed using molecular docking analysis with PfDHFR enzyme and heme unit. The docking results indicated that the necessary interactions (Asp54 and Ile14) and docking score (−9.63 to −7.36 kcal/mmol) were comparable to WR99210 (−9.89 kcal/mol). From these results nine molecules were selected for synthesis. In vitro analysis of these synthesized compounds reveal that out of the nine molecules, eight show antimalarial activity in the range of 0.61–7.55 μM for PfD6 strain and 0.43–8.04 μM for PfW2 strain. Further, molecular dynamics simulations were performed on the most active molecule to establish comparative binding interactions of these compounds and reference ligand with Plasmodium falciparum dihydrofolate reductase (PfDHFR).