Changes in Intestinal Microbiota of Type 2 Diabetes in Mice in Response to Dietary Supplementation With Instant Tea or Matcha

• Published in: Canadian journal of diabetes 2020-02, Vol.44 (1), p.44-52
• Main Authors: Zhang, Hai-hua, PhD, Liu, Jun, MD, Lv, Yang-jun, MD, Jiang, Yu-lan, MD, Pan, Jun-xian, MD, Zhu, Yue-jin, Huang, Mei-gui, Zhang, Shi-kang, PhD
• Format: Article
• Language: English
• Subjects: 16S rDNA sequencing; diabetes; diabète; Endocrinology and Metabolism; gut microbiota; microbiote intestinal; Other; séquençage du gène ARNr 16S; tea; thé
• ISSN: 1499-2671; 2352-3840
• DOI: 10.1016/j.jcjd.2019.04.021

AbstractObjectiveGut microbiota plays a key role in metabolism and health in diabetes patients with gastrointestinal microbiota dysbiosis. Thus, regulating the ecological balance of gut microbiota may provide a pathway toward improvement for these patients. Our previous study showed that functional ingredients in tea may inhibit cornstarch digestion in vitro. MethodsA cornstarch–tea diet was developed, and in this study we investigated the effects of such a diet on blood glucose and gut microbiota in diabetic mice. ResultsDiabetes resulted in significant weight loss, hyperphagia and hyperglycemia. 16S rDNA sequencing revealed that in diabetes there is significantly increased Bacteroidaceae, Helicobacteraceae, Ruminococcaceae, Enterobacteriaceae, Rikenellaceae and Saccharibacteria_genera_incertae_sedis, and significantly decreased Lactobacillaceae, Prevotellaceae, Coriobacteriaceae, Verrucomicrobiaceae and Bifidobacteriaceae. The cornstarch‒tea diet resulted in a trend toward reduced blood glucose, with particularly increased levels of Coriobacteriaceae, Lactobacillaceae, Prevotellaceae and Bifidobacteriaceae, and decreased Bacteroidaceae, Ruminococcaceae, Helicobacteraceae and Enterobacteriaceae. ConclusionsInstant tea and matcha supplementation had beneficial effects on regulation of blood glucose and gut microbiota, reversing the changes in microbiota caused by alloxan injection. The cornstarch‒tea regulation pathway is involved in bacterium group regulation rather than single-species regulation, which suggests that cornstarch combined with tea may be used as a functional food supplement for diabetes patients.