Serum levels of ANOS1 serve as a diagnostic biomarker of gastric cancer: a prospective multicenter observational study

• Published in: Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association 2020-03, Vol.23 (2), p.203-211
• Main Authors: Kanda, Mitsuro, Suh, Yun-Suhk, Park, Do Joong, Tanaka, Chie, Ahn, Sang-Hoon, Kong, Seong-Ho, Lee, Hyuk-Joon, Kobayashi, Daisuke, Fujiwara, Michitaka, Shimada, Hideaki, Cho, BeLong, Murotani, Kenta, Kim, Hyung-Ho, Yang, Han-Kwang, Kodera, Yasuhiro
• Format: Article
• Language: English
• Subjects: Abdominal Surgery; Adaptor Proteins, Signal Transducing - blood; Adult; Aged; Aged, 80 and over; Antigens; Antigens, Neoplasm - blood; Biomarkers; Biomarkers, Tumor - blood; Cancer Research; Carcinoembryonic antigen; Case-Control Studies; Dihydropyrimidinase; Enzyme-linked immunosorbent assay; Extracellular Matrix Proteins - blood; Female; Follow-Up Studies; Gastric cancer; Gastroenterology; Humans; Male; Medicine; Medicine & Public Health; Melanoma; Melanoma-associated antigen; Middle Aged; Muscle Proteins - blood; Nerve Tissue Proteins - blood; Observational studies; Oncology; Original Article; Patients; Prognosis; Prospective Studies; Serum levels; Stomach Neoplasms - blood; Stomach Neoplasms - diagnosis; Stomach Neoplasms - surgery; Surgery; Surgical Oncology; Survival Rate
• ISSN: 1436-3291; 1436-3305
• DOI: 10.1007/s10120-019-00995-z

Background Development of high-performance serum biomarkers will likely improve treatment outcomes of patients with gastric cancer (GC). We previously identified the candidate serum markers, anosmin 1 (ANOS1), dihydropyrimidinase-like 3 (DPYSL3), and melanoma-associated antigen D2 (MAGE-D2) and evaluated their clinical significance through a single-center retrospective analysis. Here we conducted a prospective multicenter observational study aimed at validating the diagnostic performance of these potential markers. Methods We analyzed serum levels before and after surgery of the three potential biomarkers in patients with GC and healthy volunteers. Quantification of serum and GC tissue levels was performed using an ELISA. Results Area under the curve (AUC) values that discriminated patients with GC from healthy controls were − 0.7058, 0.6188, and 0.5031 for ANOS1, DPYSL3, and MAGED2, respectively. The sensitivity and specificity of the ANOS1 assay were 0.36 and 0.85, respectively. The AUC value of ANOS1 that discriminated patients with stage I GC from healthy controls was 0.7131. Serum ANOS1 levels were significantly elevated in patients with stage I GC compared with those of healthy controls (median 1179 ng/ml and 461 ng/ml, respectively, P