Discarded portions of bone marrow aspirates are a new resource for patient‐derived mesenchymal stem and stromal cells

Bone marrow‐derived mesenchymal stem and stromal cells (BM‐MSCs) protect malignant cells from chemotherapy and are important potential therapeutic targets. Isolating primary BM‐MSCs for research traditionally requires the sacrifice of valuable cell populations from within the same sample. To avoid t...

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Bibliographic Details
Published in:Pediatric blood & cancer 2019-11, Vol.66 (11), p.e27936-n/a
Main Authors: Viola, Shelton A., Aftab, Chantel
Format: Article
Language:English
Subjects:
Adipogenesis
Bone marrow
Bone Marrow Cells - cytology
Bone Marrow Examination - methods
bone marrow stromal cells
Cell Culture Techniques
Cell Division
Cell Separation - methods
Cells, Cultured
Centrifugation, Density Gradient - methods
Chemotherapy
Erythrocytes
Ficoll
Hematology
hematology/oncology
Humans
leukemia
Leukemia - pathology
Mesenchymal Stem Cells - cytology
Mesenchyme
Neoplastic Stem Cells - cytology
Oncology
Paracentesis
Pediatrics
Population studies
Stromal cells
Therapeutic applications
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Summary:Bone marrow‐derived mesenchymal stem and stromal cells (BM‐MSCs) protect malignant cells from chemotherapy and are important potential therapeutic targets. Isolating primary BM‐MSCs for research traditionally requires the sacrifice of valuable cell populations from within the same sample. To avoid this, we report here a resource for isolating patient‐derived BM‐MSCs from the red blood cell layer of ficoll gradients of bone marrow aspirates, a resource that has until now been universally discarded. This resource yields BM‐MSCs nearly identical to those obtained conventionally and includes cells with a more stem‐cell like nature. Obtaining primary BM‐MSCs in this way will likely expand opportunities to study this important cell population.
ISSN:1545-5009
1545-5017
DOI:10.1002/pbc.27936