Magnetic mesoporous silica nanocomposites with binary metal oxides core-shell structure for the selective enrichment of endogenous phosphopeptides from human saliva

• Published in: Analytica chimica acta 2019-11, Vol.1079, p.111-119
• Main Authors: Li, Yilin, Liu, Liangliang, Wu, Hao, Deng, Chunhui
• Format: Article
• Language: English
• Subjects: Animals; Caseins - chemistry; Cattle; Endogenous phosphopeptides; Humans; Magnetic binary metal oxides probe; Magnetite Nanoparticles - chemistry; Mass spectrometry; Nanocomposites - chemistry; Phosphopeptides - analysis; Proteomics - methods; Saliva - chemistry; Serum Albumin, Bovine - chemistry; Silicon Dioxide - chemistry; Size-exclusive; Titanium - chemistry; Zirconium - chemistry
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2019.06.045

Developing an effective strategy for endogenous phosphopeptides enrichment and separation is necessary in phosphopeptidomics analysis due to the serious inference caused by chaotic biological environment. In this work, a size-exclusive magnetic binary metal mesoporous nanocomposites were synthesized to capture phosphorylated peptides for mass spectrometry analysis. The novel Fe3O4@TiO2–ZrO2@mSiO2 nanocomposites possessed the merits of ordered mesoporous channels, superparamagnetism and the integration of dual affinity of Zr–O and Ti–O. Compared with single-metal centered nanocomposites (Fe3O4@TiO2@mSiO2 and Fe3O4@ZrO2@mSiO2), Fe3O4@TiO2–ZrO2@mSiO2 showing much enhanced enrichment performance towards mono- and multi-phospho-peptides with better sensitivity. With all the advances, the Fe3O4@TiO2–ZrO2@mSiO2 was also successfully applied to capture endogenous phosphorylated peptides from human saliva. And consequently, a total of 30 phosphopeptides containing 2 multi- and 28 mono-phosphopeptides were identified efficiently. These results show that the novel materials have the great potential in peptidome analysis. [Display omitted] •Profiling of phosphopeptidome play an important role in the discovery of disease biomarkers.•The novel Fe3O4@TiO2–ZrO2@mSiO2 affinity probe with binary metal oxides was synthesized facilely.•The nanocomposites showed prominent enrichment performance for endogenous phosphopeptides.•The nanocomposites were successfully employed to capture endogenous phosphopeptides from human saliva.