Effects of Bisphenol A on redox balance in red blood and sperm cells and spermatic quality in zebrafish Danio rerio

• Published in: Ecotoxicology (London) 2019-10, Vol.28 (8), p.913-922
• Main Authors: Silveira, C. R., Varela Junior, A. S., Corcini, C. D., Soares, S. L., Anciuti, A. N., Kütter, M. T., Martínez, P. E.
• Format: Article
• Language: English
• Subjects: Analysis; Animals; Benzhydryl Compounds - toxicity; Bisphenol A; Blood cells; Breeding success; Damage; Danio rerio; DNA; DNA fragmentation; Earth and Environmental Science; Ecology; Ecotoxicology; Endocrine Disruptors - toxicity; Environment; Environmental Management; Epoxy resins; Erythrocytes; Erythrocytes - drug effects; Ethylenediaminetetraacetic acid; Exposure; Fish; Flow cytometry; Fluidity; Freshwater fishes; Male; Membrane fluidity; Mitochondria; Organelles; Organelles - drug effects; Oxidation-Reduction; Oxidative stress; Oxidoreductions; Parameters; Phenols; Phenols - toxicity; Reactive Oxygen Species - metabolism; Reproduction; Semen Analysis - veterinary; Sperm; Spermatozoa; Spermatozoa - drug effects; Vertebrates; Water Pollutants, Chemical - toxicity; Zebrafish; Zebrafish - physiology
• ISSN: 0963-9292; 1573-3017
• DOI: 10.1007/s10646-019-02091-5

Bisphenol-A (BPA) is a potential endocrine disruptor besides being associated with oxidative damage in several vertebrate classes. In the present study we investigated oxidative effects in erythrocytes and sperm cells as well as spermatic quality in Danio rerio exposed to 14 days at BPA concentrations of 2, 10 and 100 μg/L. Organelles structure, reactive species of oxygen (ROS) and lipoperoxidation (LPO) on erythrocytes and sperm cells were measured by flow cytometry and spermatic parameters were analyzed by the computer-assisted sperm analysis (CASA) system. For both cell types, when compared with control BPA treatment induced a significant increase in ROS and LPO production causing the membrane fluidity disorder, loss of membrane integrity and mitochondrial functionality. Furthermore, it was found a significant increase in DNA fragmentation in erythrocytes of zebrafish BPA exposed. Regarding the spermatic quality, results showed lower sperm motility in animals exposed to BPA, and alterations on velocity parameters of spermatozoa. Thus, the present study concludes that BPA affects the oxidative balance of both cell types, and that can directly affects the reproductive success of the adult Danio rerio . The sensitivity of erythrocytes to oxidative damage induced by BPA was similar to sperm cells, indicating a potential use of blood cells as indicators of oxidative damage present in fish sperm.