Photobiomodulation induces hypotensive effect in spontaneously hypertensive rats

• Published in: Lasers in medical science 2020-04, Vol.35 (3), p.567-572
• Main Authors: Buzinari, Tereza C., de Moraes, Thiago F., Cárnio, Evelin C., Lopes, Luciana A., Salgado, Helio C., Rodrigues, Gerson J.
• Format: Article
• Language: English
• Subjects: Animals; Blood pressure; Blood Pressure - radiation effects; Dentistry; Electrocardiography; Femoral artery; Femur; Heart rate; Heart Rate - radiation effects; Hemodynamics; Hemodynamics - radiation effects; Hypertension; Hypertension - blood; Hypertension - physiopathology; Hypertension - radiotherapy; Implantation; Irradiance; Irradiation; Laser applications; Lasers; Light therapy; Low-Level Light Therapy; Male; Medical materials; Medicine; Medicine & Public Health; Nitric oxide; Nitric Oxide - blood; Optical Devices; Optics; Original Article; Parameters; Photonics; Polyethylene; Polyethylenes; Quantum Optics; Rats; Rats, Inbred SHR; Recording; Rodents; Tubes
• ISSN: 0268-8921; 1435-604X
• DOI: 10.1007/s10103-019-02849-7

To evaluate whether acute photobiomodulation can elicit a hypotensive effect in spontaneously hypertensive rats (SHR). Male SHR were submitted to the implantation of a polyethylene cannula into the femoral artery. After 24 h, baseline measurements of the hemodynamic parameters: systolic, diastolic, and mean arterial pressure, and heart rate were accomplished for 1 h. Afterwards, laser application was simulated, and the hemodynamic parameters were recorded for 1 h. In the same animal, the laser was applied at six different positions of the rat’s abdomen, and the hemodynamic parameters were also recorded until the end of the hypotensive effect. The irradiation parameters were red wavelength (660 nm); average optical power of 100 mW; 56 s per point (six points); spot area of 0.0586 cm 2 ; and irradiance of 1.71 W/cm 2 yielding to a fluency of 96 J/cm 2 per point. For measuring plasma NO levels, blood was collected before the recording, as well as immediately after the end of the mediated hypotensive effect. Photobiomodulation therapy was able to reduce the systolic arterial pressure in 69% of the SHR submitted to the application, displaying a decrease in systolic, diastolic, and mean arterial pressure. No change in heart rate was observed. Nevertheless, there was an increase in serum nitric oxide levels in the SHR responsive to photobiomodulation. Our results suggest that acute irradiation with a red laser at 660 nm can elicit a hypotensive effect in SHR, probably by a mechanism involving the release of NO, without changing the heart rate.