Simultaneous determination of six components in commercial Roukou Wuwei pills using ultra‐high‐performance liquid chromatography with diode‐array detector

An efficient ultra‐performance liquid chromatography with diode‐array detector method was established for simultaneous determination of six active components in Roukou Wuwei pills, namely gallic acid, piperine, costundide, dehydrocostus lactone, isoalantolactone and alantolactone. Chromatographic se...

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Bibliographic Details
Published in:Biomedical chromatography 2019-12, Vol.33 (12), p.e4677-n/a
Main Authors: Wang, Jin, Zhang, Hai‐hong, Tian, Jia‐ming, Liu, Feng‐xi, Zhang, Yu‐ping, Zhao, Yong‐ming
Format: Article
Language:English
Subjects:
active components
Alkaloids - analysis
Benzodioxoles - analysis
Chromatography, High Pressure Liquid - methods
Drugs, Chinese Herbal - analysis
Drugs, Chinese Herbal - chemistry
Gallic Acid - analysis
Lactones - analysis
Limit of Detection
Linear Models
Piperidines - analysis
Polyunsaturated Alkamides - analysis
quality control
Reproducibility of Results
Roukou Wuwei pills
Sesquiterpenes - analysis
ultra‐performance liquid chromatography
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Summary:An efficient ultra‐performance liquid chromatography with diode‐array detector method was established for simultaneous determination of six active components in Roukou Wuwei pills, namely gallic acid, piperine, costundide, dehydrocostus lactone, isoalantolactone and alantolactone. Chromatographic separation of six components was successfully achieved on an Waters BEH C18 column (50 × 2.1 mm, 1.7 μm) with a mobile phase composed of acetonitrile and water using a gradient elution. Gallic acid and piperine were detected at 270 nm and 343 nm, respectively; while costundide, dehydrocostus lactone, isoalantolactone and alantolactone were simultaneously measured at 225 nm. All six calibration curves showed good linearity (R2 ≥ 0.9994) between the peak area of each component and corresponding concentration. Relative standard deviations for inter‐ and intra‐day precisions were
ISSN:0269-3879
1099-0801
DOI:10.1002/bmc.4677