Tanshinone IIA Can Inhibit Angiotensin II-Induced Proliferation and Autophagy of Vascular Smooth Muscle Cells via Regulating the MAPK Signaling Pathway

• Published in: Biological & pharmaceutical bulletin 2019/11/01, Vol.42(11), pp.1783-1788
• Main Authors: Lu, Jingping, Shan, Jinjun, Liu, Ning, Ding, Yao, Wang, Pei
• Format: Article
• Language: English
• Subjects: Abietanes - pharmacology; Angiotensin; Angiotensin II; Angiotensin II (Ang II); Angiotensin II - metabolism; Animals; Apoptosis; Autophagy; Autophagy - drug effects; Cell proliferation; Cell Proliferation - drug effects; Cells, Cultured; Flow cytometry; Kinases; MAP kinase; MAP Kinase Signaling System - drug effects; mitogen-activated protein kinase (MAPK); Muscle, Smooth, Vascular - drug effects; Muscle, Smooth, Vascular - metabolism; Muscles; p38 Mitogen-Activated Protein Kinases - metabolism; Phagocytosis; Phosphorylation; Primary Cell Culture; Protein kinase; Rats; Rats, Sprague-Dawley; Signal transduction; Smooth muscle; tanshinone IIA; vascular smooth muscle cell (VSMC)
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.b19-00053

To examine the effect of tanshinone IIA on Angiotensin II (Ang II)-induced proliferation and autophagy in vascular smooth muscle cells (VSMCs) and the related mechanism. VSMCs were treated with Ang II with or without tanshinone IIA (1, 5 and 10 µg/mL), and the proliferation, apoptosis in cells with different treatment were examined by methylthiazolyl tetrazolium (MTT) and flow cytometry methods. Moreover, the expression of autophagy related proteins and mitogen-activated protein kinase (MAPK) signaling molecules were examined by RT-quantitative (q)PCR and Western blot methods. Ang II induced significantly increase in the proliferation and autophagy of VSMCs, and the MAPK signaling was activated. Tanshinone IIA can attenuate Ang II-induced effects via down-regulating the MAPK signaling pathway. Tanshinone IIA can inhibit Ang II-induced proliferation and autophagy of VSMCs via regulating the MAPK signaling pathway.