Investigating the level of DNA double‐strand break in human spermatozoa and its relation to semen characteristics and IVF outcome using phospho‐histone H2AX antibody as a biomarker

Background Sperm DNA fragmentation and its relation to conventional semen parameters are well studied. However, there is limited information regarding the rate of DNA double‐strand breaks (DSBs) and its correlation to basic semen parameters and IVF outcome. Objectives The present study aimed to inve...

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Bibliographic Details
Published in:Andrology (Oxford) 2020-03, Vol.8 (2), p.421-426
Main Authors: Coban, O., Serdarogullari, M., Yarkiner, Z., Serakinci, N.
Format: Article
Language:English
Subjects:
Adult
Biomarkers - analysis
Deoxyribonucleic acid
DNA
DNA Breaks, Double-Stranded
DNA double‐strand breaks
Female
Fertilization in Vitro
Histones - analysis
Humans
ICSI
Infertility, Male - genetics
IVF
Live Birth
Male
male infertility
Middle Aged
Morphology
Motility
Pregnancy
Prospective Studies
Semen
Sperm
sperm DNA damage
Spermatozoa
γH2AX
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Summary:Background Sperm DNA fragmentation and its relation to conventional semen parameters are well studied. However, there is limited information regarding the rate of DNA double‐strand breaks (DSBs) and its correlation to basic semen parameters and IVF outcome. Objectives The present study aimed to investigate the rate of DNA DSBs in human spermatozoa and its correlation to basic semen parameters and IVF outcome. Materials and methods The prospective study includes 60 assisted reproductive treatment cycles (52 autologous and eight donors) in which the semen profiles and sperm DNA DSBs have been assessed. The level of sperm DNA DSBs in each sample has been evaluated by using a method to detect histone H2AX phosphorylation. The results were compared with basic semen values and IVF outcomes. Results No significant correlation was observed between phospho‐histone H2AX (γH2AX) levels and basic semen parameters such as semen volume (p = 0.129), sperm count (p = 0.454), total motility (p = 0.934), progressive motility (p = 0.314) and normal sperm morphology (p = 0.720). Similarly, the mean values of γH2AX did not differ with regard to the age of male participants (p = 0.300). However, cycles that resulted in live birth exhibited lower levels of γH2AX (p = 0.007). Accordingly, the level of γH2AX (p 
ISSN:2047-2919
2047-2927
DOI:10.1111/andr.12689