Origins of the enhanced affinity of RNA-protein interactions triggered by RNA phosphorodithioate backbone modification

The well-characterized interaction between the MS2 coat protein and its cognate RNA hairpin was used to evaluate changes in affinity as a result of phosphorodithioate (PS2) replacing phosphate by biolayer interferometry (BLI). A structure-based analysis of the data provides insights into the origins...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2017-09, Vol.53 (76), p.10508-10511
Main Authors: Yang, Xianbin, Dinuka Abeydeera, N, Liu, Feng-Wu, Egli, Martin
Format: Article
Language:English
Subjects:
Capsid Proteins - chemistry
chemical reactions
coat proteins
interferometry
Levivirus - chemistry
moieties
phosphates
Phosphates - chemistry
RNA
RNA - chemistry
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Summary:The well-characterized interaction between the MS2 coat protein and its cognate RNA hairpin was used to evaluate changes in affinity as a result of phosphorodithioate (PS2) replacing phosphate by biolayer interferometry (BLI). A structure-based analysis of the data provides insights into the origins of the enhanced affinity of RNA-protein interactions triggered by the PS2 moiety.
ISSN:1359-7345
1364-548X
1364-548X
DOI:10.1039/c7cc05722a