Exopolysaccharides from Aspergillus terreus: Production, chemical elucidation and immunoactivity

• Published in: International journal of biological macromolecules 2019-10, Vol.139, p.654-664
• Main Authors: Costa, Claudia R.L. de M., Menolli, Rafael A., Osaku, Erica F., Tramontina, Robson, de Melo, Renan H., do Amaral, Alex E., Duarte, Péricles A.D., de Carvalho, Mariana Mazetto, Smiderle, Fhernanda R., Silva, José L. da C., Mello, Rosiane G.
• Format: Article
• Language: English
• Subjects: Animals; Aspergillus - chemistry; beta-Glucans - chemistry; Carbohydrates polymers; Carbon - metabolism; Culture Media - chemistry; Fermentation; Gas Chromatography-Mass Spectrometry; Glucose - chemistry; Glycogen - chemistry; Hydrogen-Ion Concentration; Interleukin-1 - metabolism; Macrophage activation; Macrophages - metabolism; Macrophages, Peritoneal - metabolism; Magnetic Resonance Spectroscopy; Male; Mannans - chemistry; Methylation; Mice; Nitric Oxide - chemistry; Optimization; Polysaccharides - pharmacology; Spectroscopy, Fourier Transform Infrared; Superoxides - metabolism; Temperature; Tumor Necrosis Factor-alpha - metabolism
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2019.08.039

Aspergillus terreus, a fungus commonly used in pharmaceutical industry to produce lovastatin and other secondary metabolites, has been reported to have beneficial biological properties. In this study the exopolysaccharides (AT-EPS) produced by A. terreus were evaluated as potential modulators of certain functions of macrophages. The production parameters for EPS obtained from the liquid culture broth of the studied fungus were optimized using response surface methodology (RSM) and indicated good correlation between the experimental and predicted values. The optimum conditions for AT-EPS extraction included fermentation at 28 °C, pH 8.79, under 98 rpm of agitation, using 2.39% glucose (carbon source) and 0.957% ammonium nitrate (nitrogen source). Under these optimized conditions, AT-EPS production was 1.34 g/L medium. The chemical analyses showed that AT-EPS was composed by mannose (Man; 40.5 mol%), galactose (Gal; 35.2 mol%), and glucose (Glc; 24.3 mol%), and the spectroscopic (FTIR; NMR) and methylation analyses indicated the presence of galactomannans, β-1,3-glucans, and glycogen-like glucans. AT-EPS was tested on murine macrophages to verify its immunoactivity and the treated cells were able to produce nitric oxide, superoxide anion, TNF-α and interleukin 6 similarly to the positive control cells. Furthermore, the macrophages treated with AT-EPS showed activated-like morphological alterations. [Display omitted] •Aspergillus terreus produces exopolysaccharides in an optimized form with glucose at 2.39%, NH4NO3 at 0.957% and pH 8.79.•The EPS produced by A. terreus are constituted by galactomannans, β-1,3-glucans and glycogen-like glucans.•The EPS produced by A. terreus was able to stimulate murine macrophages to secrete NO, TNF-α and IL-1 in significant amounts.