Antibacterial Silver-Conjugated Magnetic Nanoparticles: Design, Synthesis and Bactericidal Effect

• Published in: Pharmaceutical research 2019-10, Vol.36 (10), p.147-12, Article 147
• Main Authors: Shatan, Anastasiia B., Venclíková, Kristýna, Zasońska, Beata A., Patsula, Vitalii, Pop-Georgievski, Ognen, Petrovský, Eduard, Horák, Daniel
• Format: Article
• Language: English
• Subjects: Analysis; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antibacterial activity; Antibacterial agents; Atomic absorption analysis; Atomic absorption spectroscopy; Atomic beam spectroscopy; Bacteria; Bacterial infections; Biochemistry; Biomedical and Life Sciences; Biomedical Engineering and Bioengineering; Biomedicine; Disinfection; E coli; Escherichia coli; Escherichia coli - drug effects; Hydrolysis; Iron oxides; Light scattering; Magnetic measurement; Magnetic properties; Magnetic saturation; Magnetite Nanoparticles - chemistry; Magnetization; Medical instruments; Medical Law; Monounsaturated fatty acids; Nanoparticles; Oleic acid; Oleic Acid - chemistry; Packaging; Particle Size; Pharmacology/Toxicology; Pharmacy; Photon correlation spectroscopy; Purification; Research Paper; Silanes - chemistry; Silica; Silicon dioxide; Silicon Dioxide - chemistry; Silver; Silver - chemistry; Silver - pharmacology; Silver nitrate; Spectral analysis; Staphylococcus aureus; Staphylococcus aureus - drug effects; Surface Properties; Thermal decomposition; Toxicity; Water purification; Water treatment
• ISSN: 0724-8741; 1573-904X
• DOI: 10.1007/s11095-019-2680-x

Purpose The aim was to design and thoroughly characterize monodisperse Fe 3 O 4 @SiO 2 -Ag nanoparticles with strong antibacterial properties, which makes them a candidate for targeting bacterial infections. Methods The monodisperse Fe 3 O 4 nanoparticles were prepared by oleic acid-stabilized thermal decomposition of Fe(III) oleate; the particles were coated with silica shell using a water-in-oil reverse microemulsion, involving hydrolysis and condensation of tetramethyl orthosilicate. Resulting Fe 3 O 4 @SiO 2 particles were modified by (3-mercaptopropyl)trimethoxysilane to introduce 1.1 mmol SH/g. Finally, the Fe 3 O 4 @SiO 2 -SH nanoparticles were decorated with silver nanoclusters formed by reduction of silver nitrate with NaBH 4 . The particles were analyzed by FTIR, X-ray photoelectron and atomic absorption spectroscopy, dynamic light scattering and vibrating sample magnetometry. The antibacterial activity of the Fe 3 O 4 @SiO 2 and Fe 3 O 4 @SiO 2 -Ag nanoparticles was tested against Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli bacteria cultivated on Luria agar plates or in Luria broth. Results The superparamagnetic Fe 3 O 4 @SiO 2 -Ag nanoparticles (21 nm in diameter; saturation magnetization 26 A∙m 2 /kg) were successfully obtained and characterized. Inhibitory and toxic effects against bacteria were documented by incubation of the Fe 3 O 4 @SiO 2 -Ag nanoparticles with Staphylococcus aureus and Escherichia coli . Conclusions The combination of magnetic properties together with bactericidal effects is suitable for the disinfection of medical instruments, water purification, food packaging, etc.