Evaluation of metalloproteinases-2, -9, and -13 post photobiomodulation in mice talocrural joint

• Published in: Lasers in medical science 2020-04, Vol.35 (3), p.633-640
• Main Authors: Abduch, Thais Fraga, da Silva, Pierre Augusto Victor, de Souza, Álvaro Carneiro, dos Anjos, Lúcia Mara Januário, de Souza da Fonseca, Adenilson, de Paoli, Flávia
• Format: Article
• Language: English
• Subjects: Animals; Cartilage; Cartilage (articular); Cartilage, Articular - metabolism; Cell differentiation; Collagenase 3; Connective tissues; Continuous radiation; Dentistry; Differentiation (biology); Extracellular matrix; Extracellular Matrix - metabolism; Gelatinase A; Gelatinase B; Irradiation; Joints - enzymology; Joints - radiation effects; Lasers; Levels; Light therapy; Low-Level Light Therapy; Male; Matrix Metalloproteinase 13 - genetics; Matrix Metalloproteinase 13 - metabolism; Matrix Metalloproteinase 2 - genetics; Matrix Metalloproteinase 2 - metabolism; Matrix Metalloproteinase 9 - genetics; Matrix Metalloproteinase 9 - metabolism; Matrix metalloproteinases; Medicine; Medicine & Public Health; Mice; Mice, Inbred C57BL; mRNA; Optical Devices; Optics; Original Article; Photonics; Proteins; Quantum Optics; RNA, Messenger - genetics; RNA, Messenger - metabolism; Statistics; Tissue inhibitor of metalloproteinase 2; Tissue Inhibitor of Metalloproteinase-2 - metabolism
• ISSN: 0268-8921; 1435-604X
• DOI: 10.1007/s10103-019-02860-y

The extracellular matrix (ECM) is the main constituent of connective tissue with structural and regulatory functions, stimulating cell differentiation and proliferation. Moreover, ECM is a dynamic structure in the constant remodeling process, which is controlled by a balance between metalloproteinases (MMPs) and their inhibitors (TIMPs). Photobiomodulation (PBM) is widely described in the literature and applied in clinical practices, although its effects on ECM have not yet been elucidated. Therefore, it was evaluated if PBM could alter ECM components, such as MMP-2, -9, -13, and TIMP-2 from mice talocrural joints. Mice were divided into 3 groups ( n  = 6): control, PBM 3 J cm −2 , and PBM 30 J cm −2 . A low-level laser (830 nm, 10 mW, 0.05 irradiated area, energy densities 3 J cm −2 and 30 J cm −2 , the irradiation time of 15 and 150 s, respectively, continuous wave) was applied on the joint for 4 consecutive days. mRNA levels of metalloproteinases genes (MMP-2, MMP-9, and MMP-13), their regulator (TIMP-2), and protein expressions of MMP-13 and TIMP-2 were quantified. PBM can alter only mRNA relative levels of MMP-2 at 30 J cm −2 ( p   0.05). Regarding protein expressions, MMP-13 demonstrated positive-labeled cells, only in articular cartilage, although the cell quantification did not demonstrate statistical differences when compared with the control group ( p  > 0.05). TIMP-2 did not present positive-labeled cells for any tissues evaluated. Our results indicate that PBM can alter MMP-2 mRNA relative level but cannot alter MMP-9, MMP-13, and TIMP mRNA relative levels. Moreover, both MMP-13 and TIMP-2 proteins were also unaltered after PBM.