Differential regulation of protein synthesis by skeletal muscle type in chickens

• Published in: General and comparative endocrinology 2019-12, Vol.284, p.113246-113246, Article 113246
• Main Authors: Saneyasu, Takaoki, Nakano, Yuma, Tsuchii, Nami, Kitashiro, Ayana, Tsuchihashi, Tatsuya, Kimura, Sayaka, Honda, Kazuhisa, Kamisoyama, Hiroshi
• Format: Article
• Language: English
• Subjects: Akt; Animals; Avian Proteins - biosynthesis; Chicken; Chickens - metabolism; Fasting; Fasting - metabolism; IGF-1; Insulin; Insulin-Like Growth Factor I - metabolism; Male; Muscle, Skeletal - metabolism; Phosphorylation; Protein Biosynthesis; Protein synthesis; Proto-Oncogene Proteins c-akt - metabolism; RNA, Messenger - metabolism
• ISSN: 0016-6480; 1095-6840
• DOI: 10.1016/j.ygcen.2019.113246

•Fasting inhibited protein synthesis in three different types of muscles in chicken.•The Akt/S6 pathway was inhibited by fasting in the pectoralis muscle.•Similarly, fasting inhibited the Akt/S6 pathway in the adductor profound muscle.•In the adductor superficialis muscle, fasting did not change Akt phosphorylation. In mammalian skeletal muscles, protein synthesis rates vary according to fiber types. We herein demonstrated differences in the regulatory mechanism underlying the protein synthesis in the pectoralis major (a glycolytic twitch muscle), adductor superficialis (an oxidative twitch muscle), and adductor profound (a tonic muscle) muscles of 14-day-old chickens. Under ad libitum feeding conditions, protein synthesis is significantly higher in the adductor superficialis muscle than in the pectoralis major muscle, suggesting that protein synthesis is upregulated in oxidative muscles in chickens, similar to that in mammals. In the pectoralis major muscle, fasting significantly inhibited the Akt/S6 pathway and protein synthesis with a corresponding decrease in plasma insulin concentration. Conversely, the insulin like growth factor-1 (IGF-1) mRNA levels significantly increased. These findings suggest that the insulin/Akt/S6 pathway plays an important role in the regulation of protein synthesis in the pectoralis major muscle. Interestingly, protein synthesis in the adductor superficialis muscle appears to be regulated in an Akt-independent manner, because fasting significantly decreased S6 phosphorylation and protein synthesis without affecting Akt phosphorylation. In the adductor profound muscle, IGF-1 expression, phosphorylation of Akt and S6, and protein synthesis were decreased by fasting, suggesting that insulin and/or skeletal IGF-1 appear contribute to protein synthesis via the Akt/S6 pathway. These findings revealed the differential regulation of protein synthesis depending on skeletal muscle types in chickens.