Altered expression of circular RNA in primary Sjögren’s syndrome

Objectives This study evaluated expression of circRNA in primary Sjögren’s syndrome (pSS) patients so as to find novel biomarkers for pSS screening and discussed possible role of circRNA in pSS. We also evaluated expression profile of circRNA in systemic lupus erythematosus (SLE) patients. Methods M...

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Bibliographic Details
Published in:Clinical rheumatology 2019-12, Vol.38 (12), p.3425-3433
Main Authors: Su, Lin-Chong, Xu, Wang-Dong, Liu, Xiao-Yan, Fu, Lu, Huang, An-Fang
Format: Article
Language:English
Subjects:
Adult
Biomarkers
Biomarkers - blood
Case-Control Studies
Circular RNA
Etiology
Female
Humans
Laboratories
Lupus
Male
Medicine
Medicine & Public Health
Middle Aged
miRNA
Original Article
Rheumatology
RNA, Circular - blood
Sjogren's syndrome
Sjogren's Syndrome - blood
Systemic lupus erythematosus
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Summary:Objectives This study evaluated expression of circRNA in primary Sjögren’s syndrome (pSS) patients so as to find novel biomarkers for pSS screening and discussed possible role of circRNA in pSS. We also evaluated expression profile of circRNA in systemic lupus erythematosus (SLE) patients. Methods Microarray analysis detected circRNA expression in PBMCs from five paired pSS, SLE patients, and controls. Then, differentially expressed circRNAs were validated in 30 pSS patients as compared to 30 SLE patients, healthy controls. CircRNAs interacting with miRNAs were discussed by Arraystar’s homemade miRNA target prediction software. ROC analysis assessed the diagnostic value. Results We identified 234 differentially expressed circRNAs in pSS patients and verified five selected circRNAs (including hsa_circRNA_001264, hsa_circRNA_104121, hsa_circRNA_045355, hsa_circRNA_103461, hsa_circRNA_105034). Expression of hsa_circRNA_001264, hsa_circRNA_104121, and hsa_circRNA_045355 was strongly related to some clinical, laboratory parameters, and disease activity index in pSS patients. ROC analysis indicated potential diagnostic ability for the three circRNAs in pSS patients. One hundred and forty-eight circRNAs were differently expressed between lupus patients and controls. Conclusion This study provides evidence that hsa_circRNA_001264, hsa_circRNA_104121, and hsa_circRNA_045355 might be biomarkers for pSS, correlate with pSS etiology. Key Points • Many circRNAs were dysregulated in pSS patients. • Differentially expressed circRNAs correlated with pSS clinical, laboratory features. • CircRNAs may be biomarkers for pSS.
ISSN:0770-3198
1434-9949
DOI:10.1007/s10067-019-04728-6