The needle in the haystack: Histology of post-mortem computed tomography guided biopsies versus autopsy derived tissue

• Published in: Forensic science international 2019-09, Vol.302, p.109882-109882, Article 109882
• Main Authors: Latten, Bartholomeus G.H., Bakers, Franciscus C.H., Hofman, Paul A.M., zur Hausen, Axel, Kubat, Bela
• Format: Article
• Language: English
• Subjects: Adult; Aged; Aged, 80 and over; Autopsies; Autopsy; Biopsy; Biopsy, Needle - methods; Bronchopneumonia; Computed tomography; Conflicts of interest; Consent; Cytopathology; Female; Forensic Pathology; Forensic science; Forensic sciences; Histology; Humans; Imaging techniques; Intellectual property; Internal medicine; Investigations; Liver; Liver - diagnostic imaging; Liver - pathology; Liver Diseases - diagnosis; Lung - diagnostic imaging; Lung - pathology; Lung Diseases - diagnosis; Lungs; Male; Medicine; Middle Aged; Pathology; Physicians; Postmortem; Predictive Value of Tests; Prospective Studies; Radiology; Sensitivity and Specificity; Statistical analysis; Tissues; Tomography, X-Ray Computed
• ISSN: 0379-0738; 1872-6283
• DOI: 10.1016/j.forsciint.2019.109882

•This is the first publication solely on the histological concordance of biopsies.•Postmortem CT-guided needle biopsies have a limited value.•Bronchopneumonia was the number one missed diagnosis in biopsies.•An autopsy is the method of choice in the forensic and clinical setting. The last decennia autopsies have been declining worldwide, whereas non-invasive methods have been proposed as an alternative for the conventional autopsy. Some of these methods add needle biopsies to imaging techniques to provide an alternative to histological examination of autopsy-derived tissue. The aim of this study was to investigate the representativeness of the histology of computed tomography (CT) -guided post-mortem needle biopsies in comparison to autopsy derived tissue from the same organ. Consent of next of kin was obtained from relatives of deceased within the department of Internal Medicine to perform an autopsy, postmortem CT and CT-guided needle biopsies. The lungs and the liver were routinely sampled with CT-guided postmortem biopsies and during the autopsy. In some cases extra CT-guided biopsies of lesions reported on the CT were sampled. The biopsy and the autopsy reports were independently reported and retrospectively coded according to the Nationwide Network and Registry of Histo- and Cytopathology in the Netherlands (PALGA). Three pathologists with an interest in autopsy pathology and three physicians of the department of internal medicine separately and independently interpreted all histological results in relation to the cause of death as formulated in the autopsy report. Fleiss’s Kappa was calculated and a consensus grade was defined. Fleiss’s Kappa showed substantial agreement in both lungs and moderate agreement in the liver. Of the 60 included cases 44% of the CT-guided postmortem biopsies in the left lung and 30% in the right lung showed false negative findings, primarily concerning a bronchopneumonia. In contrast, 91% of the liver biopsies showed concordant results, however only 22% of all liver biopsies concerned a major diagnosis related to the cause of death. The positive predictive value of the biopsies of the left lung, right lung and the liver were respectively 86.6%, 88.8% and 100%. The negative predictive values of these biopsies were 32.4%, 50% and 92.7%. CT-guided postmortem biopsies of the lungs have a mediocre predictive value. Due to a low prevalence of relevant findings the overall usefulness of biopsies of the liver are limited. Convent