Polymorphism eNOS Glu298Asp modulates the inflammatory response of human peripheral blood mononuclear cells

• Published in: Cytokine (Philadelphia, Pa.) Pa.), 2020-01, Vol.125, p.154812-154812, Article 154812
• Main Authors: Maurer, Patrícia, Barbisan, Fernanda, Azzolin, Veronica Farina, Berro, Lyana Feijoo, Montagner, Renata, Duarte, Marta Maria Medeiros Frescura, da Cruz, Ivana Beatrice Mânica, Manfredini, Vanusa, Piccoli, Jacqueline da Costa Escobar
• Format: Article
• Language: English
• Subjects: African Continental Ancestry Group - genetics; Afrodescendant; Alleles; Cytokines - metabolism; eNOS; Genetic Association Studies; Genotype; Humans; Inflammation; Inflammation - genetics; Inflammation - metabolism; Interleukin-1 - metabolism; Interleukin-6 - metabolism; Leukocytes, Mononuclear - drug effects; Leukocytes, Mononuclear - immunology; Leukocytes, Mononuclear - metabolism; Mitogens - pharmacology; Negroid race; Nitric Oxide - metabolism; Nitric Oxide Synthase Type III - genetics; Phenotype; Phytohemagglutinins - pharmacology; Polymorphism, Single Nucleotide; Tumor Necrosis Factor-alpha - metabolism
• ISSN: 1043-4666; 1096-0023
• DOI: 10.1016/j.cyto.2019.154812

[Display omitted] •Glu298Asp influences the inflammatory response of PBMCs of black people.•Allele T of SNP Glu298Asp carries greater susceptibility to inflammation.•Genotype GG presented higher levels of IL10 and nitric oxide.•Allele G protect against inflammation.•Antihypertensive and anti-inflammatory therapy may be influenced by GLP298asp SNP. Nitric oxide is a gaseous radical produced by the nitric oxide endothelial synthase (eNOS) whose most studied physiological action is the vasodilation. However, it also acts in the defense of the organism through the formation of cytotoxic radicals, which can potentiate the inflammatory lesion of the cells. The Glu298Asp is a single nucleotide polymorphism (SNP) in the eNOS gene related to the risk of cardiovascular disease. Blacks present a higher prevalence of hypertension and cardiovascular mortality. Then, we aimed to evaluate the influence of Glu298Asp polymorphism on inflammatory response in vitro and gene expression in blacks. Peripheral blood mononuclear cells (PBMC) from blacks with different Glu298Asp genotypes were treated with phytohemagglutinin (PHA), a mitogen and activator of T cells. Oxidative, inflammatory markers, and expression of inflammation genes were evaluated. The genotype frequencies were TT 6.7%; TG 29.3% and GG 64.0%. Activation of PBMCs with 125 μg of PHA modulated the expression of inflammatory genes and increased levels of inflammatory cytokines. The T allele showed increased susceptibility to inflammation (higher levels of interleukin 1, interleukin 6 and tumor necrosis factor alpha; p