Lysosomal dysfunction disturbs porcine oocyte maturation and developmental capacity by disorganizing chromosome/cytoskeleton and activating autophagy/apoptosis

Lysosome, an important organelle in eukaryotes, can sequester macromolecules submitted by the endocytosis and autophagy pathways for degradation and recycling. Massive macromolecular turnover is also vital to the growth and development of mammalian oocytes. However, the functional role of lysosomes...

Full description

Saved in:
Bibliographic Details
Published in:Theriogenology 2019-12, Vol.140, p.44-51
Main Authors: Miao, Jia-Kun, Liu, Yun-Hua, Liu, Shuai, Liu, Xiao-Man, Wang, Pei-Chao, Du, Zhi-Qiang, Yang, Cai-Xia
Format: Article
Language:English
Subjects:
Animals
Apoptosis - drug effects
Apoptosis - physiology
Autophagy
Autophagy - drug effects
Autophagy - physiology
Blastocyst
Chloroquine - pharmacology
Chromosomes - metabolism
Chromosomes - ultrastructure
Cytoskeleton - drug effects
Cytoskeleton - metabolism
Cytoskeleton - ultrastructure
In Vitro Oocyte Maturation Techniques - methods
In Vitro Oocyte Maturation Techniques - veterinary
Lysosome
Lysosomes - drug effects
Lysosomes - physiology
Meiosis - drug effects
Oocyte
Oocytes - cytology
Oocytes - drug effects
Oocytes - growth & development
Porcine
Reactive Oxygen Species - metabolism
Swine - embryology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Lysosome, an important organelle in eukaryotes, can sequester macromolecules submitted by the endocytosis and autophagy pathways for degradation and recycling. Massive macromolecular turnover is also vital to the growth and development of mammalian oocytes. However, the functional role of lysosomes in the meiotic maturation of mammalian oocytes remains largely unexplored. Here, by treating in vitro matured porcine cumulus-oocyte complexes (COCs) with chloroquine (CQ), a lysosome inhibitor, we showed that regardless of CQ concentration, lysosomal inhibition affected neither the extrusion of the first polar body (PB1), nor the ROS levels. However, CQ treatment dramatically decreased the rates of oocytes with normal chromosome alignment and cytoskeleton organization (P 
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2019.08.019