Association of CTLA‐4, TNF alpha and IL 10 polymorphisms with susceptibility to hepatocellular carcinoma

• Published in: Scandinavian journal of immunology 2019-12, Vol.90 (6), p.e12819-n/a
• Main Authors: Wang, Jia‐jia, Wang, Zhi‐bin, Tan, Tai‐chang
• Format: Article
• Language: English
• Subjects: Adult; Aged; Alleles; Carcinoma, Hepatocellular - genetics; Carcinoma, Hepatocellular - metabolism; Carcinoma, Hepatocellular - pathology; Case-Control Studies; Cell proliferation; CTLA-4 Antigen - genetics; CTLA‐4; Cytokines; Cytokines - metabolism; Cytotoxicity; Cytotoxicity, Immunologic; DNA sequencing; Female; Gene Frequency; genetic polymorphisms; Genetic Predisposition to Disease; Genotype; Genotypes; Hepatocellular carcinoma; Humans; Immunoregulation; Interleukin 1; Interleukin 10; Interleukin-10 - genetics; Leukocytes (mononuclear); Liver cancer; Liver Neoplasms - genetics; Liver Neoplasms - metabolism; Liver Neoplasms - pathology; Lymphocyte Activation - immunology; Lymphocytes; Lymphocytes T; Male; Middle Aged; Neoplasm Staging; Peripheral blood mononuclear cells; Polymorphism, Single Nucleotide; Statistical analysis; T-Lymphocytes - immunology; T-Lymphocytes - metabolism; Tumor necrosis factor; Tumor Necrosis Factor-alpha - genetics
• ISSN: 0300-9475; 1365-3083
• DOI: 10.1111/sji.12819

Our aim was to evaluate the association of genetic polymorphisms of immunoregulatory molecules with susceptibility to hepatocellular carcinoma (HCC). The polymorphisms in CTLA‐4 (−318 T/C, CT60 G/A), TNF (−238 G/A, −308 G/A) and IL10 (−592 C/A, −819 C/T) were genotyped by PCR and DNA sequencing. The functional relevance of the polymorphisms was examined by ELISAs, in vitro lymphocyte proliferation assay and cytotoxic assay. The CTLA‐4 −318 TC/TT, CTLA‐4 CT60 GG, IL10 −592 CA and −819 CT/TT variants, CTLA‐4 −318 T and IL 10 −819 T alleles were positively associated with HCC risk (P