A novel electrochemical sensor for determination of DNA damage biomarker (8-hydroxy-2′-deoxyguanosine) in urine using sonochemically derived graphene oxide sheets covered zinc oxide flower modified electrode

• Published in: Ultrasonics sonochemistry 2019-11, Vol.58, p.104622-104622, Article 104622
• Main Authors: Govindasamy, Mani, Wang, Sea-Fue, Subramanian, Bowya, Ramalingam, R. Jothi, Al-lohedan, Hamad, Sathiyan, Anandraj
• Format: Article
• Language: English
• Subjects: 8-Hydroxy-2'-Deoxyguanosine - chemistry; 8-Hydroxy-2'-Deoxyguanosine - urine; Biomarkers - chemistry; Biomarkers - urine; Catalysis; DNA Damage; DNA damage biomarker; Electrochemistry - instrumentation; Electrodes; Graphene oxide nanosheets; Graphite - chemistry; Limit of Detection; Sonochemical synthesis; Ultrasonic Waves; Urinalysis - instrumentation; Urine real sample; Zinc Oxide - chemistry; Zinc oxide nanoflower
• ISSN: 1350-4177; 1873-2828
• DOI: 10.1016/j.ultsonch.2019.104622

•Facile synthesis of Transition Metal Oxides based ZnO NFs@GOS nanocomposite have been developed.•The ZnO NFs@GOS was applied to the non-enzymatic biosensor of oxidative stress biomarker.•The nanocomposite is exhibits high selectivity and sensitivity.•The nanocomposite is exhibits nanomolar LOD. To explore a novel and multi-layer based graphene oxide covered zinc oxide nanoflower (ZnO NFs@GOS) as a modified electrode materials by sonochemical technique (40 kHz, 300 W). Herein, novel nanocomposite is successfully characterized by various characterization analysis (FESEM, HRTEM, XRD, XPS and (EIS) electrochemical impedance spectroscopy) and employed as high sensitive modified electrode (ZnO NFs@GOS nanocomposite) for the electrochemical determination of biomarker. 8-hydroxy-2′-deoxyguanosine (8-HDG) is one of the important cancer and oxidative stress biomarker. The results demonstrated that the ZnO NFs@GOS modified SPCE reveal well-defined electro-oxidation peak at 0.36 V (vs. Ag/AgCl). The high sensitive properties of the optimized flower like modified electrode are because of the excellent synergistic effect of the ZnO flower and the graphene oxide nanosheets, as evidenced by a superior bio-sensing performance. The nanocomposite fabricated modified biosensor was facilitating the analysis of 8-HDG in the concentration ranges of 0.05–536.5 µM with a low detection limit is 8.67 nM. The ZnO NFs@GOS modified sensor can also employed for the determination of 8-HDG in human urine samples, promising its application towards the quantification of cancer biomarker in biological samples.