LncRNA CTC-497E21.4 promotes the progression of gastric cancer via modulating miR-22/NET1 axis through RhoA signaling pathway

Background Long non-coding RNAs (lncRNAs) have emerged as important roles in gastric cancer (GC). However, the role of the dysregulated lncRNAs in GC remained large unknown. We investigated the clinical significance, biological function and mechanism of CTC-497E21.4 in GC. Methods Firstly, RTFQ-PCR...

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Bibliographic Details
Published in:Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association 2020-03, Vol.23 (2), p.228-240
Main Authors: Zong, Wei, Feng, Wei, Jiang, Yun, Cao, Yaning, Ke, Yuchen, Shi, Xin, Ju, Shaoqing, Cong, Hui, Wang, Xudong, Cui, Ming, Jing, Rongrong
Format: Article
Language:English
Subjects:
Abdominal Surgery
Animals
Apoptosis
Biomarkers, Tumor - genetics
Biomarkers, Tumor - metabolism
Cancer Research
Case-Control Studies
Cell Cycle
Cell Proliferation
Disease Progression
Female
Follow-Up Studies
Gastric cancer
Gastroenterology
Gene Expression Regulation, Neoplastic
Gene regulation
Humans
Kinases
Localization
Lymph nodes
Lymphatic Metastasis
Male
Medicine
Medicine & Public Health
Metastases
Mice
Mice, Nude
MicroRNAs - genetics
Middle Aged
Neoplasm Invasiveness
Non-coding RNA
Oncogene Proteins - genetics
Oncogene Proteins - metabolism
Oncology
Original Article
Plasmolysis
Prognosis
rhoA GTP-Binding Protein - genetics
rhoA GTP-Binding Protein - metabolism
RhoA protein
RNA, Long Noncoding - genetics
Signal Transduction
Stomach Neoplasms - genetics
Stomach Neoplasms - metabolism
Stomach Neoplasms - pathology
Surgical Oncology
Survival Rate
Tumor Cells, Cultured
Tumorigenesis
Xenograft Model Antitumor Assays
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Summary:Background Long non-coding RNAs (lncRNAs) have emerged as important roles in gastric cancer (GC). However, the role of the dysregulated lncRNAs in GC remained large unknown. We investigated the clinical significance, biological function and mechanism of CTC-497E21.4 in GC. Methods Firstly, RTFQ-PCR was used to detect the expression of CTC-497E21.4 in GC. Furthermore, knockdown of CTC-497E21.4 was conducted to assess the effect of CTC-497E21.4 in vitro and vivo. Subcellular localization of CTC-497E21.4 was determined by nuclear plasmolysis PCR and FISH. We also predicted CTC-497E21.4 binding miRNAs and downstream target genes and evaluated its regulation of miR-22 by acting as a ceRNA. Result CTC-497E21.4 was upregulated in GC tissues and GC cell lines ( P  
ISSN:1436-3291
1436-3305
DOI:10.1007/s10120-019-00998-w